Breukelman H J, Beintema J J, Confalone E, Costanzo C, Sasso M P, Carsana A, Palmieri M, Furia A
Dipartimento di Chimica Organica e Biologica, Università di Napoli, Italy.
J Mol Evol. 1993 Jul;37(1):29-35. doi: 10.1007/BF00170459.
Mammalian pancreatic ribonucleases form a family of homologous proteins that has been extensively investigated. The primary structures of these enzymes were used to derive phylogenetic trees. These analyses indicate that the presence of three strictly homologous enzymes in the bovine species (the pancreatic, seminal, and cerebral ribonucleases) is due to gene duplication events which occurred during the evolution of ancestral ruminants. In this paper we present evidence that confirms this finding and that suggests an overall structural conservation of the putative ribonuclease genes in ruminant species. We could also demonstrate that the sequences related to ox ribonuclease coding regions present in genomic DNA of the giraffe species are the orthologues of the bovine genes encoding the three ribonucleases mentioned above.
哺乳动物的胰腺核糖核酸酶构成了一个已被广泛研究的同源蛋白家族。这些酶的一级结构被用于推导系统发育树。这些分析表明，牛物种中存在三种严格同源的酶（胰腺、精液和脑核糖核酸酶）是由于在反刍动物祖先的进化过程中发生的基因复制事件。在本文中，我们提供的证据证实了这一发现，并表明反刍动物物种中假定的核糖核酸酶基因在整体结构上具有保守性。我们还能够证明，长颈鹿物种基因组DNA中与牛核糖核酸酶编码区域相关的序列是上述三种牛核糖核酸酶基因的直系同源物。
