Cirvilleri Gabriella, Spina Stefania, Iacona Calogero, Catara Antonino, Muleo Rosario
Dipartimento di Scienze e Tecnologie Fitosanitarie, Università di Catania, Italy.
J Plant Physiol. 2008 Jul 7;165(10):1107-19. doi: 10.1016/j.jplph.2008.01.009. Epub 2008 Apr 24.
The cherry rootstock 'Colt' line was transformed with a phytochrome A rice gene with the aim of altering light perception. Three transgenic events were chosen because of a modified developmental behavior. When red enriched light was supplied horizontally to stems, the PD3 transgenic line showed an increased rate of phytomer formation associated to a superior rate of plant growth compared to wild type (WT). Under the same light conditions, the PO1 and PA lines were less altered in morphology and development. When far-red enriched light was supplied, all transgenic lines had a reduced rate of growth, with the PD3 line being the most similar to the WT. The influence of the alien gene on root and leaf-associated bacteria was studied for a duration of 1 year. Significantly more culturable bacteria were recovered from PA lines than from PO1, PD3 and WT lines. On average, significantly more fluorescent pseudomonads were recovered from the rhizosphere of PA and PO1 lines than from PD3 and WT. No significant differences were detected in the number of bacteria recovered from the phyllosphere of transgenic and WT plant lines. A total of 143 Pseudomonas fluorescens strains isolated from rhizosphere of transgenic and WT lines were tested for their antagonistic activity against Phytophthora nicotianae and differences between bacteria derived from transgenic and WT were not detected. Fluorescent pseudomonads strains isolated from phyllosphere of PA and PO1 lines showed antagonistic activity against P. syringae pv. syringae, whereas no difference among the transgenic and WT lines was detected when fluorescent Pseudomonas strains were tested against P. syringae pv. mors-prunorum. Pathogenicity tests were conducted on rooted and micropropagated plants with P. s. pv. syringae and P. s. pv. mors-prunorum: in all assays, the PO1 lines were the most tolerant to P. s. pv. Syringae, and the PO1 and PD3 were tolerant to P. s. pv. mors-prunorum.
为了改变光感知，樱桃砧木“Colt”品系用一种水稻光敏色素A基因进行了转化。由于发育行为发生了改变，选择了三个转基因事件。当向茎水平提供富含红光的光时，与野生型（WT）相比，PD3转基因品系显示出节间形成速率增加，与更高的植物生长速率相关。在相同光照条件下，PO1和PA品系在形态和发育上变化较小。当提供富含远红光的光时，所有转基因品系的生长速率都降低，其中PD3品系与野生型最为相似。研究了外源基因对根和叶相关细菌的影响，为期1年。从PA品系中回收的可培养细菌明显多于PO1、PD3和WT品系。平均而言，从PA和PO1品系根际回收的荧光假单胞菌明显多于PD3和WT品系。在转基因和野生型植株叶际回收的细菌数量上未检测到显著差异。从转基因和野生型品系根际分离出的总共143株荧光假单胞菌菌株对烟草疫霉的拮抗活性进行了测试，未检测到转基因和野生型来源细菌之间的差异。从PA和PO1品系叶际分离出的荧光假单胞菌菌株对丁香假单胞菌丁香致病变种具有拮抗活性，而当荧光假单胞菌菌株对李痘假单胞菌进行测试时，转基因和野生型品系之间未检测到差异。用丁香假单胞菌丁香致病变种和李痘假单胞菌对生根和微繁殖植株进行了致病性测试：在所有试验中，PO1品系对丁香假单胞菌丁香致病变种最具耐受性，PO1和PD3对李痘假单胞菌具有耐受性。
