Lamas-Ardisana Pedro José, Queipo Paula, Fanjul-Bolado Pablo, Costa-García Agustín
Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Asturias, Spain.
Anal Chim Acta. 2008 May 12;615(1):30-8. doi: 10.1016/j.aca.2008.03.034. Epub 2008 Mar 25.
Carboxylated multiwalled carbon nanotubes (MWCNT-COOH) were used to modify the working electrode surface of different screen-printed electrodes. The effect of this modification on the electrodic characteristics (double layer capacitance, electroactive area and heterogeneous rate constants for the electron transfer) was evaluated and optimized for the cyclic voltammetric determination of p-aminophenol. The enzymatic hydrolysis of p-aminophenylphosphate was employed for the quantification of alkaline phosphatase, one of the most important label enzymes in immunoassays. Finally, ELISA assays were carried out to quantify pneumolysin using this enzymatic system. Results obtained indicated that low superficial densities of MWCNT-COOH (0.03-0.06 microg mm(-2)) yielded the same electrodic improvements but with better analytical properties.
羧基化多壁碳纳米管（MWCNT-COOH）用于修饰不同丝网印刷电极的工作电极表面。评估了这种修饰对电极特性（双层电容、电活性面积和电子转移的异相速率常数）的影响，并针对对氨基苯酚的循环伏安测定进行了优化。采用对氨基苯磷酸的酶促水解来定量碱性磷酸酶，碱性磷酸酶是免疫分析中最重要的标记酶之一。最后，使用该酶促系统进行酶联免疫吸附测定（ELISA）以定量肺炎球菌溶血素。获得的结果表明，低表面密度的MWCNT-COOH（0.03 - 0.06微克毫米(-2)）产生了相同的电极改进效果，但具有更好的分析性能。
