Hernández F, Coto E, Colmenares A, Moreira L
Departamento de Microbiología, Facultad de Microbiología, Universidad de Costa Rica, San José.
Rev Biol Trop. 1991 Jun;39(1):177-80.
Biological particulate specimens, including Saccharomyces cerevisiae yeast, bovine spermatozoa and human blood cells (normal erythrocytes and leukemic cells) were processed for scanning and transmission electron microscopy using the coagulated plasma technique. The specimens were suspended in frozen and thawed plasma; later, coagulation was induced by adding CaCl2. The clot was cut into small pieces and processed as tissue fragments. The technique is an useful tool when processing biological particulate specimens for electron microscopy.
使用凝固血浆技术对包括酿酒酵母、牛精子和人类血细胞（正常红细胞和白血病细胞）在内的生物颗粒标本进行扫描电子显微镜和透射电子显微镜检查处理。将标本悬浮于冻融血浆中；随后，通过添加氯化钙诱导凝血。将凝块切成小块，并作为组织碎片进行处理。该技术是处理用于电子显微镜检查的生物颗粒标本时的一种有用工具。
