Kálmán E, Haklik L, Eke A, Kovács P, Pálinkás G
Acta Biol Acad Sci Hung. 1976;27(2-3):177-82.
A microchamber was developed for the examination of biological specimens in nearly natural environments, in an electron microscope, at 70kV accelerating voltage.. The chamber can be supplied continually with the sample and with the reagents, which makes it suitable for the study of biochemical reactions, too. Temperature and vapour pressure in the chamber can be controlled and the thickness of the specimen can be varied. Transmission electron micrographs of biological specimens, such as human blood cells, bull gametes and Bacillus subtilis have been obtained. Mobility of microorganisms, which is regarded as a criterion of the wet state, has been observed.
研制了一种微室，用于在近自然环境中，在70kV加速电压下的电子显微镜中检查生物标本。该微室可以持续供应样品和试剂，这使其也适用于生化反应的研究。微室内的温度和蒸汽压可以控制，标本的厚度也可以变化。已获得了生物标本的透射电子显微照片，如人类血细胞、公牛配子和枯草芽孢杆菌。还观察到了微生物的移动性，这被视为湿润状态的一个标准。
