[Genetic variation of Anopheles dirus A and D (Diptera:Culicidae) in China: inferred by mtDNA-CO I gene sequences].

OBJECTIVE

To interpret genetic variation and population structure of Anopheles dirus A and D from China by molecular marker.

METHODS

Samples included An. dirus A of Hainan laboratory colony (n=13), and field specimen from Mengla (n=17) and Jiangcheng (n=17) in Yunnan Province. The specimens were identified by PCR assay before study, mtDNA-CO I region was amplified and sequenced. Genetic variation and population structure was estimated according to sequence data.

RESULTS

The mtDNA-CO I gene with a length of 959 bp was analyzed. There were three haplotypes in An. dirus A and six haplotypes in An. dirus D. The above haplotypes distributed in three populations uniformly. The average number of pairwise differences within Mengla population (7.4412) was greater than that of Jiangcheng (1.2794) and Hainan (1.0513) populations, which suggested that the level of genetic divergence was the highest within Mengla population. The result of hierarchical AMOVA estimation showed a limited geneflow (Fst=0.799 9), therefore the variation level in a population (20.01%) was smaller than among the populations (79.99%).

CONCLUSION

The inter-specific genetic variation between An. dirus A and D in China was small and the level of divergence among individuals was high.