Wang Dong, Ma Ya-jun, Zhou Hong-ning
Department of Etiologic Biology, The Second Military Medical University, Shanghai 200433, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2007 Oct;25(5):368-71, 375.
To interpret genetic variation and population structure of Anopheles dirus A and D from China by molecular marker.
Samples included An. dirus A of Hainan laboratory colony (n=13), and field specimen from Mengla (n=17) and Jiangcheng (n=17) in Yunnan Province. The specimens were identified by PCR assay before study, mtDNA-CO I region was amplified and sequenced. Genetic variation and population structure was estimated according to sequence data.
The mtDNA-CO I gene with a length of 959 bp was analyzed. There were three haplotypes in An. dirus A and six haplotypes in An. dirus D. The above haplotypes distributed in three populations uniformly. The average number of pairwise differences within Mengla population (7.4412) was greater than that of Jiangcheng (1.2794) and Hainan (1.0513) populations, which suggested that the level of genetic divergence was the highest within Mengla population. The result of hierarchical AMOVA estimation showed a limited geneflow (Fst=0.799 9), therefore the variation level in a population (20.01%) was smaller than among the populations (79.99%).
The inter-specific genetic variation between An. dirus A and D in China was small and the level of divergence among individuals was high.
通过分子标记解析中国多斑按蚊A和D型的遗传变异及群体结构。
样本包括海南实验室种群的多斑按蚊A（n = 13），以及来自云南勐腊（n = 17）和江城（n = 17）的野外样本。在研究前通过PCR检测对样本进行鉴定，扩增并测序线粒体DNA-CO I区域。根据序列数据估计遗传变异和群体结构。
分析了长度为959 bp的线粒体DNA-CO I基因。多斑按蚊A有3种单倍型，多斑按蚊D有6种单倍型。上述单倍型在3个群体中均匀分布。勐腊群体内成对差异的平均数（7.4412）大于江城（1.2794）和海南（1.0513）群体，这表明勐腊群体内的遗传分化水平最高。分层AMOVA估计结果显示基因流有限（Fst = 0.799 9），因此群体内的变异水平（20.01%）小于群体间的变异水平（79.99%）。
中国多斑按蚊A和D型之间的种间遗传变异较小，个体间的分化水平较高。
