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在低葡萄糖无细胞培养基中培养克氏锥虫上鞭毛体,会改变其分化为循环后期锥鞭毛体的能力。

Cultivation of Trypanosoma cruzi epimastigotes in low glucose axenic media shifts its competence to differentiate at metacyclic trypomastigotes.

作者信息

De Lima Ana R, Navarro María C, Arteaga Rosa Y, Contreras Víctor T

机构信息

Laboratorio de Protozoología, Centro BioMolP, Universidad de Carabobo, Bárbula, Valencia, Estado Carabobo, Venezuela.

出版信息

Exp Parasitol. 2008 Jul;119(3):336-42. doi: 10.1016/j.exppara.2008.03.003. Epub 2008 Mar 15.

Abstract

This study offers an insight into why Trypanosoma cruzi epimastigotes lose their capacity to differentiate into metacyclic forms, if maintained in culture media long-term through serial passages. The biological and metabolic behaviour of two T. cruzi strains isolated from various origins (human, opossum), and maintained under two schedules (alternate triatomine/mouse passages and serial culture media) were compared. To determine the effect of the environment on the parasites, the epimastigotes were grown under extreme conditions (high and low glucose concentrations), and the glucose consumption, ammonia production and changes in pH, either in one compartment (along the growth curve) or two compartments (induced metacyclogenesis) were compared. The glucose effect on the stages involved in metacyclogenesis at antigenic level was also evaluated. The results indicate that T. cruzi adapts to various environmental conditions and also that the ability of epimastigotes to undergo metacyclogenesis are influenced by the maintenance schedule. Antigenic profile analysis supports the idea that epimastigotes adapted to culture media do not complete their molecular differentiation into the trypomastigote metacyclic stage. These transition forms conserve some degree of gene expression of the epimastigote stage.

摘要

本研究深入探讨了克氏锥虫前鞭毛体如果通过连续传代在培养基中长期培养,为何会丧失分化为循环后期形式的能力。比较了从不同来源(人类、负鼠)分离并在两种培养方案(交替经锥蝽/小鼠传代和连续在培养基中培养)下维持的两种克氏锥虫菌株的生物学和代谢行为。为了确定环境对寄生虫的影响,将前鞭毛体在极端条件(高葡萄糖浓度和低葡萄糖浓度)下培养,并比较了在一个隔室(沿生长曲线)或两个隔室(诱导循环后期发育)中的葡萄糖消耗、氨生成及pH变化。还评估了葡萄糖在抗原水平对参与循环后期发育各阶段的影响。结果表明,克氏锥虫能适应各种环境条件,并且前鞭毛体进行循环后期发育的能力受培养方案的影响。抗原谱分析支持这样的观点,即适应培养基的前鞭毛体并未完成向循环后期锥鞭毛体阶段的分子分化。这些过渡形式保留了前鞭毛体阶段一定程度的基因表达。

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