甘蓝夜蛾变态过程中arylphorin的命运及可能作用。

The fate and possible role of arylphorin during the metamorphosis of Mamestra brassicae.

Sass M, Low P, Móricz Z, Csikós G, Kovács J

Department of General Zoology, Eötvös Loránd University, Budapest, Hungary.

Acta Biol Hung. 1991;42(1-3):141-60.

Abstract

Arylphorin, one of the storage proteins has been isolated from the hemolymph of Mamestra brassicae. 2. It has been established that Mamestra arylphorin is the most similar to manducin from among the known storage proteins of other species. 3. A rabbit polyclonal antibody has been developed against arylphorin, and its concentration changes have been determined quantitatively by ELISA in the hemolymph and fat body from the 1st day of the last larval instar to the 3rd day of the imago stage. 4. Histological sections were made on each day during the investigated period and it was shown by immunohistochemical methods that the main quantity of arylphorin was accumulated in the storage protein granules of the fat body and it could be detected even in the imaginal fat body. 5. The uptake of arylphorin by the fat body is induced by 20-hydroxyecdysone. 6. During differentiation of the imaginal cuticle arylphorin is incorporated first in the epidermal cells and it is built in the endocuticular layer of the integument thereafter.

摘要

芳基脂蛋白是一种储存蛋白，已从甘蓝夜蛾的血淋巴中分离出来。2. 已确定甘蓝夜蛾芳基脂蛋白与其他物种已知储存蛋白中的咽下腺蛋白最为相似。3. 已制备出针对芳基脂蛋白的兔多克隆抗体，并通过酶联免疫吸附测定法定量测定了从末龄幼虫第1天到成虫期第3天血淋巴和脂肪体中其浓度的变化。4. 在研究期间的每一天制作组织切片，免疫组织化学方法显示，芳基脂蛋白的主要量积累在脂肪体的储存蛋白颗粒中，甚至在成虫脂肪体中也能检测到。5. 20-羟基蜕皮酮可诱导脂肪体摄取芳基脂蛋白。6. 在成虫表皮分化过程中，芳基脂蛋白首先被整合到表皮细胞中，随后被整合到体壁的内皮层中。