Sass M, Kömüves L G, Kovács J, Feuer L
Dept. Gen. Zool., Eötvös Univ., Budapest, Hungary.
Acta Biol Hung. 1986;37(3-4):249-57.
Correlative changes in the protein contents of haemolymph and fat body and the accumulation of protein storage granules in the fat body cells of Mamestra brassicae were investigated during the last larval stage in normally developing larvae and following administration of glutaurine (1 X 10(-4) mg/g body weight). The protein content of the haemolymph of untreated larvae increased up to the 4th day of the stage, declined during days 5 and 6, and increased again before pupation. In the glutaurine-treated larvae the amount of proteins in the haemolymph was as high as in the controls during the first four days but continued to rise up to the end of the stage. The protein content of the fat body started to increase from the 3rd day and heavy accumulation of protein storage granules in the cells of fat body was observed on the 5th and following days. The protein content of the fat body of glutaurine-treated larvae remained at a low level and the protein storage granules were absent in the cells. The inhibition of the selective uptake of haemolymphatic storage proteins by fat body following glutaurine treatment is suggested.
研究了正常发育幼虫最后一个幼虫阶段以及给予谷氨酰胺（1×10⁻⁴毫克/克体重）后，甘蓝夜蛾血淋巴和脂肪体蛋白质含量的相关变化以及脂肪体细胞中蛋白质储存颗粒的积累情况。未处理幼虫的血淋巴蛋白质含量在该阶段的第4天前增加，在第5天和第6天下降，并在化蛹前再次增加。在谷氨酰胺处理的幼虫中，血淋巴中的蛋白质含量在前四天与对照组一样高，但在该阶段结束前持续上升。脂肪体的蛋白质含量从第3天开始增加，在第5天及之后的几天观察到脂肪体细胞中蛋白质储存颗粒大量积累。谷氨酰胺处理的幼虫脂肪体蛋白质含量保持在低水平，细胞中没有蛋白质储存颗粒。这表明谷氨酰胺处理后脂肪体对血淋巴储存蛋白的选择性摄取受到抑制。
