Terpos Evangelos, Sarantopoulos Angelos, Kouramba Anna, Katsarou Olga, Stavropoulos John, Masouridi Stavroula, Karafoulidou Anastasia, Meletis John
Department of Medical Research, 251 General Air Force Hospital, Athens, Greece.
Med Sci Monit. 2008 May;14(5):CR276-280.
Anemia is a common feature in HIV infection. An increased sensitivity of lymphocytes from HIV patients to lysis by complement has been correlated with a decreased expression of CD55 and CD59 in their surface. The aim of this study was to evaluate CD55/CD59 presence in red cells of HIV patients and explore possible correlations with clinical parameters.
MATERIAL/METHODS: CD55/CD59 expression was evaluated in erythrocytes of 37 patients (30M/7F, median age: 39 years) with HIV infection (25 also having hemophilia), 121 controls, and 8 PNH patients using the sephacryl-gel microtyping system. Ham and sucrose tests were also performed.
Anemia was present in 14/37 (37%) HIV patients. Interestingly, all HIV patients had deficient CD55 and/or CD59 erythrocytes: 8 (21%) for both CD55 andCD59 and 29 (78%) isolated CD55 and/or CD59 negativity. Deficient erythrocytes did not account for more than 10% of the total in the vast majority of patients. In controls, only 2 (1%) had red cells with double CD55/CD59 negativity and 3 (2%) had isolated deficiency. All PNH patients had a simultaneous CD55/CD59 deficiency. Positive Ham and sucrose tests were found only in PNH. There was no correlation between the presence of deficient CD55/CD59 erythrocytes and anemia, hemolysis, antiretroviral therapy, CD4+ counts, viral load, or concomitant hepatitis C infection in HIV patients.
This study provides evidence supporting the presence of erythrocytes with CD55 and/or CD59 deficiency in HIV. Further studies using molecular techniques will be required to clarify the exact role of this deficiency in HIV patients.
贫血是HIV感染的常见特征。HIV患者淋巴细胞对补体介导的裂解敏感性增加与它们表面CD55和CD59表达降低有关。本研究的目的是评估HIV患者红细胞中CD55/CD59的存在情况，并探讨其与临床参数的可能相关性。
材料/方法：使用sephacryl凝胶微定型系统评估37例HIV感染患者（30例男性/7例女性，中位年龄：39岁）（其中25例还患有血友病）、121例对照者和8例阵发性睡眠性血红蛋白尿症（PNH）患者红细胞中的CD55/CD59表达。还进行了酸溶血试验和蔗糖溶血试验。
14/37（37%）的HIV患者存在贫血。有趣的是，所有HIV患者都有CD55和/或CD59缺陷的红细胞：8例（21%）CD55和CD59均缺陷，29例（78%）单独存在CD55和/或CD59阴性。在绝大多数患者中，缺陷红细胞占总数的比例不超过10%。在对照者中，只有2例（1%）红细胞同时存在CD55/CD59阴性，3例（2%）存在单独缺陷。所有PNH患者均同时存在CD55/CD59缺陷。仅在PNH患者中发现酸溶血试验和蔗糖溶血试验阳性。HIV患者中，CD55/CD59缺陷红细胞的存在与贫血、溶血抗逆转录病毒治疗、CD4+细胞计数、病毒载量或合并丙型肝炎感染之间无相关性。
本研究提供了证据支持HIV患者中存在CD55和/或CD59缺陷的红细胞。需要进一步使用分子技术的研究来阐明这种缺陷在HIV患者中的确切作用。
