Vigueras Gabriel, Shirai Keiko, Martins de Souza Daniel, Franco Telma Teixeira, Fleuri Luciana Francisco, Revah Sergio
Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco no. 186, México, DF, México.
Appl Microbiol Biotechnol. 2008 Aug;80(1):147-54. doi: 10.1007/s00253-008-1490-6. Epub 2008 Apr 29.
Paecilomyces lilacinus consumed toluene as the sole carbon source in a gas-phase biofilter packed with perlite obtaining an average elimination capacity of 50 g m(-3) h(-1), a removal efficiency of 53%, and a final biomass of 31.6 mg biomass g dry support(-1). Hydrophobin proteins from the mycelium produced in the biofilter were purified by formic acid extraction and precipitated by electrobubbling, and the molecular weight was found to be 10.6 +/- 0.3 kDa. The peptide mass fingerprinting analysis of the purified hydrophobin by matrix-assisted laser desorption/ionization time-of-flight resulted in the identification of two peptides that presented high homology with sequences of class I hydrophobin proteins from other ascomycetous fungi when compared against the National Center for Biotechnology Information database. The yield of hydrophobin (PLHYD) from P. lilacinus was 1.1 mg PLHYD g biomass(-1). These proteins modified the hydrophobicity of Teflon by lowering the contact angle from 130.1 (+/-2) degrees to 57.0 (+/-5) degrees supporting hot sodium dodecyl sulfate washing. This work is the first report about biodegradation of toluene by the nematophagous fungus P. lilacinus in a gas-phase biofilter and the identification of its hydrophobin protein.
淡紫拟青霉在填充珍珠岩的气相生物滤池中以甲苯作为唯一碳源，平均去除能力为50 g m(-3) h(-1)，去除效率为53%，最终生物量为31.6 mg生物量 g干载体(-1)。生物滤池中产生的菌丝体的疏水蛋白通过甲酸提取进行纯化，并通过电泡沉淀，发现其分子量为10.6 +/- 0.3 kDa。通过基质辅助激光解吸/电离飞行时间对纯化的疏水蛋白进行肽质量指纹分析，与美国国立生物技术信息中心数据库比对后，鉴定出两种与其他子囊菌的I类疏水蛋白序列具有高度同源性的肽。淡紫拟青霉的疏水蛋白（PLHYD）产量为1.1 mg PLHYD g生物量(-1)。这些蛋白质通过将特氟龙的接触角从130.1（+/-2）度降低到57.0（+/-5）度，改变了特氟龙的疏水性，支持热十二烷基硫酸钠洗涤。这项工作是关于食线虫真菌淡紫拟青霉在气相生物滤池中对甲苯进行生物降解及其疏水蛋白鉴定的首次报道。
