Pothiratana Chetsada, Fuangsawat Wasapon, Jintapattanakit Anchalee, Teerapatsakul Churapa, Thachepan Surachai
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Center for Advanced Studies in Tropical Natural Resources, NRU-KU, Kasetsart University, Bangkok, Thailand.
Mycology. 2020 Aug 31;12(1):58-67. doi: 10.1080/21501203.2020.1804474.
Hydrophobin proteins were extracted from mycelia, the culture media (potato dextrose broth, PDB), and fruiting bodies. The putative hydrophobins obtained showed approximate sizes ranging from 8.0 to 25.0 kDa, dependent on their source. Multiple hydrophobin protein bands were detected in fruiting bodies. The hydrophobin yielded from aerial mycelia, or fruiting bodies, was approximately 6 mg/g dried weight. The crude extracts were examined for their properties in regards to surface modification, emulsification, and surface activity. Coating of hydrophobic Teflon sheet with crude extract made the surface significantly hydrophilic, whereas exposure of glass surfaces to extracts resulted in enhanced hydrophobicity. Crude extracts from culture media of displayed emulsifying activity when mixed with hexane and could significantly reduce the surface tension of 60% ethanol and deionised water. The putative hydrophobin protein band from culture media (9.6 kDa), as analysed using LC-MS/MS, contained an amino acid fragment structurally similar to class I hydrophobin proteins from Basidiomycetes.
疏水蛋白是从菌丝体、培养基（马铃薯葡萄糖肉汤，PDB）和子实体中提取的。所获得的假定疏水蛋白显示出大约8.0至25.0 kDa的大小范围，这取决于它们的来源。在子实体中检测到多个疏水蛋白条带。从气生菌丝体或子实体中产生的疏水蛋白产量约为6 mg/g干重。对粗提物的表面改性、乳化和表面活性等性质进行了检测。用粗提物包被疏水的聚四氟乙烯片会使表面显著亲水，而将玻璃表面暴露于提取物会导致疏水性增强。培养基的粗提物与己烷混合时表现出乳化活性，并且能够显著降低60%乙醇和去离子水的表面张力。使用液相色谱-串联质谱（LC-MS/MS）分析，培养基中的假定疏水蛋白条带（9.6 kDa）包含一个氨基酸片段，其结构与担子菌纲的I类疏水蛋白相似。
