Feng Bin, Shao Hua, Zhang Hao
Institute for Industrial Hygiene and Occupational Disease of Shandong Province, Jinan, 250062, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2008 Feb;26(2):86-8.
To detect the binding sites and characteristics of the adduct from the reaction of formaldehyde, acetaldehyde, acrolein with DNA.
Formaldehyde, acetaldehyde, acrolein were reacted with four kinds of deoxyribonucleoside monophosphate (dNMP) in buffered solutions with neutral pH. The reaction products were separated by high performance liquid chromatograph (HPLC) and characterized by UV spectroscopy.
The reaction of formaldehyde, acetaldehyde with dG was separated and detected by HPLC. The reaction of acrolein, formate, acetic acid, Mercapturic acid with dG was not separated and detected by HPLC, while the dominant dNMP binding with formaldehyde, acetaldehyde was also determined.
Formaldehyde, acetaldehyde could bind with dGMP to express genotoxic effects.
检测甲醛、乙醛、丙烯醛与DNA反应产物加合物的结合位点及特性。
甲醛、乙醛、丙烯醛在中性pH缓冲溶液中与四种脱氧核糖核苷单磷酸（dNMP）反应。反应产物通过高效液相色谱（HPLC）分离，并用紫外光谱进行表征。
甲醛、乙醛与dG的反应可通过HPLC分离和检测。丙烯醛、甲酸、乙酸、巯基尿酸与dG的反应不能通过HPLC分离和检测，同时还确定了与甲醛、乙醛结合的主要dNMP。
甲醛、乙醛可与dGMP结合以表现出遗传毒性作用。
