[Detection of binding of three aldehyde compounds with DNA using high performance liquid chromatograph].

OBJECTIVE

To detect the binding sites and characteristics of the adduct from the reaction of formaldehyde, acetaldehyde, acrolein with DNA.

METHODS

Formaldehyde, acetaldehyde, acrolein were reacted with four kinds of deoxyribonucleoside monophosphate (dNMP) in buffered solutions with neutral pH. The reaction products were separated by high performance liquid chromatograph (HPLC) and characterized by UV spectroscopy.

RESULTS

The reaction of formaldehyde, acetaldehyde with dG was separated and detected by HPLC. The reaction of acrolein, formate, acetic acid, Mercapturic acid with dG was not separated and detected by HPLC, while the dominant dNMP binding with formaldehyde, acetaldehyde was also determined.

CONCLUSION

Formaldehyde, acetaldehyde could bind with dGMP to express genotoxic effects.