2,2',4,4'-四溴二苯醚与2,2',4,4',5-六氯联苯联合处理对SH-SY5Y细胞的细胞遗传毒性

[Cyto-genotoxicity induced by 2, 2', 4, 4'-tetrabromodiphenyl ethers combined with 2, 2', 4, 4', 5-hexachlorobiphenyl treatment in SH-SY5Y cells].

作者信息

Xia Tao, He Wei-hong, Zhang Ming, Gao Ping, Xu Zhi-xia, Xu Ba-yi, Chen Xue-min, Wang Ai-guo

机构信息

MOE Key Lab of Environment and Health, Department of Oc-cupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2008 Feb;26(2):89-93.

PMID:18445317

Abstract

OBJECTIVE

To investigate the cyto-genotoxicity of 2, 2', 4, 4'-tetrabromodiphenyl ethers (PBDE-47) combined with 2, 2', 4, 4', 5-hexachlorobiphenyl (PCB153) treatment in SH-SY5Y cells.

METHODS

Exponentially growing SH-SY5Y cells were exposed to different concentrations of PBDE-47 or/and PCB153 for 24 h in vitro. Cell viability, DNA damage, chromosome abnormalities, and DNA-protein crosslinks (DPC) were measured using MTT, comet assay, cytokinesis-block micronucleus (CBMN) test, and SDS-KCl assay respectively.

RESULTS

Compared to the each single PBDE-47 groups, the nuclear division index (NDI) was significantly lower (P < 0.05) and the frequencies of micronuclei (MNI), percentage of DNA in the tail, Olive tail moment and DPC were significantly increased (P < 0.05) in the PBDE-47 combined with PCB153 groups. There was a statistical decrease in cell viability in groups of 4 micromol/L PBDE-47 and above combined with PCB153 than that in contrast to the same dose of PBDE-47 group or PCB153 alone (P < 0.05). Significant increase was found in MNI frequency and DPC in 2 micromol/L PBDE-47 and above combined with PCB153 than those in the single PCB153 group (P < 0.05). In the groups of 4 micromol/L PBDE-47 and above combined with PCB153, the cell NDI were significantly lower than that of the single PCB153 group (P < 0.05). Compared to the single PCB153 group, the percentage of DNA in the tail and Olive tail moment was significantly increased in the 8 micromol/L PBDE-47 combined with 5 micromol/L PCB153. Factorial analysis showed that interactions between PBDE-47 and PCB153 existed in inhibiting cell viability, inducing DNA damage, MNI, and DPC formation (P < 0.01), and possessing synergistic effects.

CONCLUSION

Some dose of PBDE-47 combined with PCB153 can inhibit cell viability, induce DNA damage, DPC formation, and chromosome abnormalities. The pattern of the combined effect is synergistic in cyto-genotoxicity.

摘要

目的

研究2,2',4,4'-四溴二苯醚（PBDE-47）与2,2',4,4',5-六氯联苯（PCB153）联合处理对SH-SY5Y细胞的细胞遗传毒性。

方法

将处于指数生长期的SH-SY5Y细胞在体外暴露于不同浓度的PBDE-47或/和PCB153 24小时。分别采用MTT法、彗星试验、胞质分裂阻滞微核（CBMN）试验和SDS-KCl试验检测细胞活力、DNA损伤、染色体异常和DNA-蛋白质交联（DPC）。

结果

与各单一组PBDE-47组相比，PBDE-47与PCB153联合处理组的核分裂指数（NDI）显著降低（P<0.05），微核频率（MNI）、尾部DNA百分比、Olive尾矩和DPC显著升高（P<0.05）。4μmol/L及以上PBDE-47与PCB153联合处理组的细胞活力较相同剂量的PBDE-47单一组或PCB153单一组显著降低（P<0.05）。2μmol/L及以上PBDE-47与PCB153联合处理组的MNI频率和DPC较PCB153单一组显著升高（P<0.05）。4μmol/L及以上PBDE-47与PCB153联合处理组的细胞NDI显著低于PCB153单一组（P<0.05）。8μmol/L PBDE-47与5μmol/L PCB153联合处理组的尾部DNA百分比和Olive尾矩较PCB153单一组显著升高。析因分析表明，PBDE-47与PCB153在抑制细胞活力、诱导DNA损伤、MNI和DPC形成方面存在相互作用（P<0.01），具有协同效应。