Department of Environmental Health and MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, People's Republic of China.
Environ Toxicol. 2010 Dec;25(6):564-72. doi: 10.1002/tox.20517.
Polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) are important recalcitrant halogenated compounds that have been regarded as major environmental pollutants. Recently, their concurrent appearance in the environment and humans and their structural and toxicological profile similarities have sparked interest in the potential toxicologic consequences of their coexposure. The aim of the current study was to evaluate the cytogenotoxic effects induced by 2,2',4,4'-tetrabromodiphenyl ether (PBDE-47) combined with 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153) treatment in human neuroblastoma cells (SH-SY5Y) in vitro. SH-SY5Y cells were exposed to different concentrations of PBDE-47 (0, 2, 4, 8 μM) with or without PCB153 (5 μM) for 24 h. Thereafter, the cell viability, DNA damage, chromosomal abnormalities, and DNA-protein crosslinks (DPC) were determined. The results show that PBDE-47 and PCB153 alone and in combination induce DNA damage, with an increase in the frequency of micronuclei (MN) and DPC formation with increasing PBDE-47 concentration. In cells coexposed to PBDE-47 and PCB153, the cell viability significantly decreased while the MN frequency, DNA damage and DPC formation were all obviously increased compared to those of cells treated with the corresponding concentrations of PBDE-47 or PCB153 alone. Factorial analysis suggests that there were interactions between PBDE-47 and PCB153. The results imply that PBDE-47 interacts with PCB153 to inhibit cell viability and induce DNA damage, DPC formation, and chromosome abnormalities.
多溴联苯醚（PBDEs）和多氯联苯（PCBs）是重要的难降解卤代化合物，已被视为主要的环境污染物。最近，它们在环境和人类中的同时出现及其结构和毒理学特征的相似性引起了人们对它们共同暴露的潜在毒理学后果的关注。本研究旨在评估 2,2',4,4'-四溴二苯醚（PBDE-47）与 2,2',4,4',5,5'-六氯联苯（PCB153）联合处理对体外人神经母细胞瘤细胞（SH-SY5Y）的细胞遗传毒性作用。将 SH-SY5Y 细胞暴露于不同浓度的 PBDE-47（0、2、4、8 μM），同时或不与 PCB153（5 μM）共同暴露 24 h。然后，测定细胞活力、DNA 损伤、染色体异常和 DNA-蛋白质交联（DPC）。结果表明，PBDE-47 单独以及与 PCB153 联合作用可诱导 DNA 损伤，随着 PBDE-47 浓度的增加，微核（MN）和 DPC 形成的频率增加。在共暴露于 PBDE-47 和 PCB153 的细胞中，细胞活力显著降低，而 MN 频率、DNA 损伤和 DPC 形成均明显高于单独用 PBDE-47 或 PCB153 处理的细胞。析因分析表明 PBDE-47 和 PCB153 之间存在相互作用。结果表明，PBDE-47 与 PCB153 相互作用抑制细胞活力并诱导 DNA 损伤、DPC 形成和染色体异常。
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