de Souza H B, Amato Neto V, Braz L M, Moreira A A, Takiguti C K, Campos R, Matsubara L
Laboratório de Investigação Médica-Parasitologia, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, Brasil.
Rev Inst Med Trop Sao Paulo. 1991 May-Jun;33(3):221-6.
In order to simplify breeding of triatominae in the laboratory, for performing xenodiagnosis and other biologic studies, we tried to feed the insects "in vitro" with citrated or defibrinated blood from commercially abated chicken. Two types of efficacy observations were carried out with Triatoma infestans: a) analysis of the chaining of successive nymphal stages, viability of satisfactory matching, fertile oviposition and adequate reproduction; b) assessment of viability and infectivity of Trypanosoma cruzi in the insects. As a conclusion, it became evident that, despite operational easiness, the objectives were not achieved, since the classical procedure used as the control, was always superior.
为了简化实验室中锥蝽的饲养，以便进行虫媒接种诊断和其他生物学研究，我们尝试用商业宰杀的鸡的枸橼酸化或去纤维蛋白血液对昆虫进行“体外”喂养。对侵袭锥蝽进行了两种类型的功效观察：a）分析连续若虫阶段的衔接、良好匹配的生存能力、可育产卵和充分繁殖；b）评估昆虫体内克氏锥虫的生存能力和感染性。作为结论，很明显，尽管操作简便，但目标并未实现，因为用作对照的传统方法总是更优。
