急性酒精灌注期间大鼠心脏细胞内游离镁的耗竭：一项31P核磁共振研究。

Depletion of intracellular free magnesium in rat hearts during acute alcohol perfusion: a 31P nuclear magnetic resonance study.

作者信息

Jelicks L A, Gupta R K

机构信息

Department of Physiology, Albert Einstein College of Medicine, Yeshiva University, Bronx, N.Y.

出版信息

Magnes Trace Elem. 1991;10(2-4):136-41.

PMID:1844546

Abstract

The effect of acute ethanol perfusion on the intracellular free magnesium level, intracellular pH, and high energy phosphate levels of isolated adult rat hearts (n = 13) were studied using 31P nuclear magnetic resonance (NMR) spectroscopy. Perfusion with 1% ethanol solution resulted in a 43% decrease in the intracellular free magnesium level in Langendorff perfused rat hearts while intracellular pH was not significantly altered. In most hearts ethanol perfusion also resulted in increased intracellular inorganic phosphate and reduced phosphocreatine and ATP levels, corresponding to a significant reduction in phosphorylation potential. The implications of these results in the pathogenesis of alcohol-induced hypertension are discussed in light of our previous studies on spontaneously hypertensive and normotensive rats which demonstrated a correlation between cardiac free magnesium levels and blood pressure.

摘要

采用31P核磁共振（NMR）光谱法研究了急性乙醇灌注对成年大鼠离体心脏（n = 13）细胞内游离镁水平、细胞内pH值和高能磷酸水平的影响。用1%乙醇溶液灌注Langendorff灌流的大鼠心脏，导致细胞内游离镁水平降低43%，而细胞内pH值无显著变化。在大多数心脏中，乙醇灌注还导致细胞内无机磷酸盐增加，磷酸肌酸和ATP水平降低，这与磷酸化电位的显著降低相对应。根据我们之前对自发性高血压大鼠和正常血压大鼠的研究结果，讨论了这些结果在酒精性高血压发病机制中的意义，该研究表明心脏游离镁水平与血压之间存在相关性。