Rossi A, Martin J, de Leiris J
J Physiol (Paris). 1980;76(8):902-5.
Continuous measurements of high energy phosphate compounds and intracellular pH in perfused, beating rat hearts, were performed by 31P nuclear magnetic resonance (NMR). Hearts were placed in a 15 mm NMR tube and perfused at 28 degrees C by conventional methods with a phosphate-free solution. Phosphorus NMR spectra were recorded at 101,3 MHz in a Brucker WP 250 spectrometer. Global mild ischemia was achieved by reducing the coronary flow to 1/10 of its initial value. Changes in creatine phosphate (CP) and inorganic phosphate (Pi) levels and intracellular pH (pHi) were monitored in the course of a 50 min ischemia and during the post-ischemic phase. When the breakdown of CP was less than 30%, the decrease in pHi was about 0.1-0.2 pH unit; for a greater CP decrease, the fall in pHi was about 1 pH unit.
采用31P核磁共振（NMR）技术，对灌注跳动的大鼠心脏中的高能磷酸化合物和细胞内pH进行连续测量。将心脏置于15毫米的NMR管中，通过常规方法在28℃下用无磷酸盐溶液进行灌注。在Brucker WP 250光谱仪中于101.3 MHz记录磷NMR光谱。通过将冠状动脉血流量减少至其初始值的1/10来实现整体轻度缺血。在50分钟的缺血过程中和缺血后阶段监测磷酸肌酸（CP）和无机磷酸盐（Pi）水平以及细胞内pH（pHi）的变化。当CP分解小于30%时，pHi下降约0.1 - 0.2个pH单位；对于CP下降幅度更大的情况，pHi下降约1个pH单位。
