腺苷掺入三磷酸腺苷：形成分隔的三磷酸腺苷。
Incorporation of adenosine into ATP: formation of compartmentalized ATP.
作者信息
Rapaport E, Zamecnik P C
出版信息
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3122-5. doi: 10.1073/pnas.73.9.3122.
Abstract
The incorporation of [3H]adenosine, [3H]adenine, and [3H]hypoxanthine into adenine nucleotides of nude (athymic) mouse liver and human hepatoma grown subcutaneously in nude mice was studied. 3H and 32P radioactive labeling in vivo of acid-soluble nucleotides followed by chromatographic procedures indicated that, in contrast to [3H]adenine and [3H]hypoxanthine, [3H]adenosine is preferentially incorporated into ATP in comparison with its incorporation into AMP and ADP. This phenomenon, as well as complementing the recently reported 3-fold increase in total cellular ATP upon treatmen- with 0.5-1.0 mM concentrations of adenosine, indicates the formation from adenosine of compartmentalized ATP that is not produced from either adenine or hypoxanthine. The observed effect is of larger magnitude in the growth-arrested normal liver than in the actively growing tumor.
摘要
研究了[3H]腺苷、[3H]腺嘌呤和[3H]次黄嘌呤掺入裸（无胸腺）鼠肝脏以及皮下接种于裸鼠的人肝癌组织腺嘌呤核苷酸的情况。体内对酸溶性核苷酸进行3H和32P放射性标记，随后采用色谱法分析表明，与[3H]腺嘌呤和[3H]次黄嘌呤相比，[3H]腺苷优先掺入ATP，而非AMP和ADP。这一现象，以及近期报道的用0.5 - 1.0 mM浓度腺苷处理后细胞内总ATP增加3倍的情况，均表明腺苷可形成分隔化的ATP，而腺嘌呤或次黄嘌呤无法产生这种ATP。在生长停滞的正常肝脏中观察到的这种效应比在活跃生长的肿瘤中更为显著。
相似文献
1
Incorporation of adenosine into ATP: formation of compartmentalized ATP.腺苷掺入三磷酸腺苷：形成分隔的三磷酸腺苷。
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3122-5. doi: 10.1073/pnas.73.9.3122.
2
3
Secretory mechanisms. Behaviour of adenine nucleotides during the platelet release reaction induced by adenosine diphosphate and adrenaline.分泌机制。二磷酸腺苷和肾上腺素诱导血小板释放反应过程中腺嘌呤核苷酸的行为。
Biochem J. 1972 Aug;129(1):67-82. doi: 10.1042/bj1290067.
4
Effects of anaerobiosis on adenine nucleotide levels and the release of ATP by Leishmania major promastigotes.无氧状态对硕大利什曼原虫前鞭毛体腺嘌呤核苷酸水平及ATP释放的影响。
Comp Biochem Physiol B. 1989;94(3):453-60. doi: 10.1016/0305-0491(89)90180-6.
5
Release of adenosine, inosine and hypoxanthine from rabbit non-myelinated nerve fibres at rest and during activity.静息和活动期间兔无髓神经纤维中腺苷、肌苷和次黄嘌呤的释放。
J Physiol. 1984 Dec;357:67-77. doi: 10.1113/jphysiol.1984.sp015489.
6
The energy metabolism of Novikoff ascites hepatoma cells. I. The effects of glucose on the intracellular level of adenine nucleotides.诺维科夫腹水肝癌细胞的能量代谢。I. 葡萄糖对腺嘌呤核苷酸细胞内水平的影响。
Can J Biochem. 1971 Jun;49(6):686-94. doi: 10.1139/o71-098.
7
8
Extracellular ATP formation on vascular endothelial cells is mediated by ecto-nucleotide kinase activities via phosphotransfer reactions.血管内皮细胞上的细胞外ATP形成是由胞外核苷酸激酶活性通过磷酸转移反应介导的。
FASEB J. 2001 Jan;15(1):251-260. doi: 10.1096/fj.00-0268com.
9
Compartmentalized ATP pools produced from adenosine are nuclear pools.由腺苷产生的分隔化ATP池是细胞核池。
J Cell Physiol. 1980 Nov;105(2):267-74. doi: 10.1002/jcp.1041050210.
10
Adenine nucleotide levels and adenosine metabolism in cultured calvarial bone.培养颅骨中的腺嘌呤核苷酸水平与腺苷代谢
Acta Physiol Scand. 1984 Apr;120(4):551-5. doi: 10.1111/j.1748-1716.1984.tb07420.x.
引用本文的文献
1
Continuous intravenous infusion of ATP in humans yields large expansions of erythrocyte ATP pools but extracellular ATP pools are elevated only at the start followed by rapid declines.在人体中持续静脉输注三磷酸腺苷（ATP）会使红细胞中的ATP储备大幅增加，但细胞外ATP储备仅在开始时升高，随后迅速下降。
Purinergic Signal. 2015 Jun;11(2):251-62. doi: 10.1007/s11302-015-9450-y. Epub 2015 Apr 29.
2
Intracellular ATP concentration contributes to the cytotoxic and cytoprotective effects of adenosine.细胞内 ATP 浓度对腺苷的细胞毒性和细胞保护作用有贡献。
PLoS One. 2013 Oct 3;8(10):e76731. doi: 10.1371/journal.pone.0076731. eCollection 2013.
3
Purine nucleotide metabolism of germinating soybean embryonic axes.发芽大豆胚轴的嘌呤核苷酸代谢。
Plant Physiol. 1979 Jan;63(1):100-4. doi: 10.1104/pp.63.1.100.
4
Responses of adenine nucleotides in germinating soybean embryonic axes to exogenously applied adenine and adenosine.萌发大豆胚轴中外源腺嘌呤和腺苷对腺嘌呤核苷酸的响应。
Plant Physiol. 1977 Nov;60(5):689-92. doi: 10.1104/pp.60.5.689.
5
Some further observations on the stimulation by high external potassium of the sodium efflux in barnacle muscle fibers.关于高细胞外钾对藤壶肌纤维钠外流刺激作用的一些进一步观察
Pflugers Arch. 1982 Dec;395(4):318-25. doi: 10.1007/BF00580796.
6
Both hypoxanthine and adenosine stimulate DNA synthesis independently in serum-starved L cells treated with platelet protein.次黄嘌呤和腺苷在用血小板蛋白处理的血清饥饿L细胞中均可独立刺激DNA合成。
Proc Natl Acad Sci U S A. 1983 Dec;80(24):7420-3. doi: 10.1073/pnas.80.24.7420.
7
A comparison of adenine and some derivatives on pig isolated tracheal muscle.腺嘌呤及其某些衍生物对猪离体气管平滑肌作用的比较。
Br J Pharmacol. 1983 Dec;80(4):603-11. doi: 10.1111/j.1476-5381.1983.tb10049.x.
8
Radioautographic visualization of differences in the pattern of [3H]uridine and [3H]orotic acid incorporation into the RNA of migrating columnar cells in the rat small intestine.放射自显影观察[3H]尿苷和[3H]乳清酸掺入大鼠小肠迁移柱状细胞RNA模式的差异。
J Cell Biol. 1984 May;98(5):1619-29. doi: 10.1083/jcb.98.5.1619.
9
Presence of diadenosine 5',5''' -P1, P4-tetraphosphate (Ap4A) in mamalian cells in levels varying widely with proliferative activity of the tissue: a possible positive "pleiotypic activator".5',5''' -P1，P4-四磷酸二腺苷（Ap4A）在哺乳动物细胞中的存在情况，其水平随组织的增殖活性而有很大差异：一种可能的正向“多效性激活剂”。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3984-8. doi: 10.1073/pnas.73.11.3984.
10
Role of adenine in pathogenesis of Mycoplasma pneumoniae infections of tracheal epithelium.腺嘌呤在气管上皮支原体肺炎感染发病机制中的作用。
Med Microbiol Immunol. 1978 May 26;165(1):43-55. doi: 10.1007/BF02121231.
本文引用的文献
1
The enzymatic synthesis of adenylic acid; adenosinekinase.腺苷酸的酶促合成；腺苷激酶。
J Biol Chem. 1951 Apr;189(2):801-14.
2
[A simple technic for extremely rapid freezing of large pieces of tissue].[一种用于极快速冷冻大块组织的简单技术]
Pflugers Arch Gesamte Physiol Menschen Tiere. 1960;270:399-412.
3
Adenosine triphosphate in liver after partial hepatectomy and acute stress.部分肝切除术后及急性应激状态下肝脏中的三磷酸腺苷
J Biol Chem. 1967 Nov 10;242(21):4963-71.
4
Nucleotide pools and [6-14C]orotic acid incorporation in early regenerating rat liver.早期再生大鼠肝脏中的核苷酸池及[6-¹⁴C]乳清酸掺入
Biochim Biophys Acta. 1966 Dec 21;129(3):445-59. doi: 10.1016/0005-2787(66)90060-8.
5
In vivo modification of the energy charge in the liver cell.肝细胞中能荷的体内修饰。
Biochem Biophys Res Commun. 1972 Feb 16;46(3):1441-5. doi: 10.1016/s0006-291x(72)80138-4.
6
The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers.作为调节参数的腺苷酸库的能荷。与反馈调节剂的相互作用。
Biochemistry. 1968 Nov;7(11):4030-4. doi: 10.1021/bi00851a033.
7
Nucleotide biosynthesis from preformed purines in mammalian cells: regulatory mechanisms and biological significance.哺乳动物细胞中由预先形成的嘌呤进行核苷酸生物合成：调控机制与生物学意义。
Prog Nucleic Acid Res Mol Biol. 1970;10:87-119. doi: 10.1016/s0079-6603(08)60562-0.
8
Lethality of adenosine for cultured mammalian cells by interference with pyrimidine biosynthesis.腺苷通过干扰嘧啶生物合成对培养的哺乳动物细胞的致死性。
J Cell Sci. 1973 Sep;13(2):429-39. doi: 10.1242/jcs.13.2.429.
9
Purine excretion by mammalian cells deficient in adenosine kinase.缺乏腺苷激酶的哺乳动物细胞的嘌呤排泄
J Cell Physiol. 1973 Jun;81(3):315-22. doi: 10.1002/jcp.1040810304.
10
Purine ribonucleotide catabolism: clinical and biochemical significance. Review.嘌呤核糖核苷酸分解代谢：临床与生化意义。综述。
Nutr Metab. 1974;16(2):65-78. doi: 10.1159/000175474.
Zotero 插件