Rapaport E
J Cell Physiol. 1980 Nov;105(2):267-74. doi: 10.1002/jcp.1041050210.
Incubation of African green monkey kidney (BS-C-1) cells and mouse fibroblasts (3T6) in the presence of adenosine for 4 hours resulted in increases in the nuclear compartment pools of adenosine 5'-triphosphate (ATP) and nuclear ATP/adenosine 5'-diphosphate (ADP) ratios. Adenine and inosine, which yield increases in total cellular ATP pools and ATP/ADP ratios similar to those promoted by adenosine, do not produce similar increases in the nuclear compartment. Adenosine-promoted increases in nuclear ATP pools were higher in the untransformed, serially propagated, BS-C-1 cells than in the spontaneously transformed 3T6 cells. Adenosine-promoted compartmentalized ATP pools in primary chick embryo fibroblasts were reduced upon transformation of these cells with Rous sarcoma virus, resulting in free mixing of all of the ATP pools synthesized from various salvage precursors. The growth regulatory properties of the nuclear compartment pools of adenine nucleotides is suggested by the big increases in nuclear ATPase and adenosine 5'-monophosphate (AMP) deaminase activities upon the entry of 3T6 cells into the S phase of their cycle. These enzymatic activities would tend to lower the nuclear ATP/ADP ratios and reduce the total adenine nucleotide pools in these nuclei respectively--conditions which were shown by earlier in vitro studies to be favorable to DNA replication.
在腺苷存在的情况下，将非洲绿猴肾（BS-C-1）细胞和小鼠成纤维细胞（3T6）孵育4小时，导致三磷酸腺苷（ATP）的核区室池以及核ATP/二磷酸腺苷（ADP）比率增加。腺嘌呤和肌苷可使总细胞ATP池和ATP/ADP比率增加，其增加情况与腺苷促进的相似，但它们不会使核区室产生类似的增加。在未转化的、连续传代的BS-C-1细胞中，腺苷促进的核ATP池增加幅度高于自发转化的3T6细胞。用劳斯肉瘤病毒转化原代鸡胚成纤维细胞后，腺苷促进的区室化ATP池减少，导致由各种补救前体合成的所有ATP池自由混合。3T6细胞进入其细胞周期的S期时，核ATP酶和5'-单磷酸腺苷（AMP）脱氨酶活性大幅增加，这表明腺嘌呤核苷酸的核区室池具有生长调节特性。这些酶活性往往会分别降低核ATP/ADP比率并减少这些细胞核中的总腺嘌呤核苷酸池——早期体外研究表明这些条件有利于DNA复制。
