Janković Teodora, Savikin Katarina, Menković Nebojsa, Aljancić Ivana, Leskovac Andreja, Petrović Sandra, Joksić Gordana
Institute for Medicinal Plant Research Josif Pancić, Belgrade, Serbia.
Planta Med. 2008 Jun;74(7):736-40. doi: 10.1055/s-2008-1074524. Epub 2008 Apr 29.
The aim of this study was to identify active principles of Gentianella austriaca responsible for the reduction of the incidence of micronuclei in irradiated lymphocytes in vitro. The radioprotective effects of ether (EF) and methanolic (MeF) fractions, water-soluble xanthones demethylbellidifolin (1), demethylbellidifolin 8-O-glucoside (2), bellidifolin 8-O-glucoside (3), and flavonoid swertisin (4) against chromosomal damage induced by gamma-rays were determined using the micronucleus test. EF and MeF showed better protection in treatment of human lymphocytes after gamma-irradiation than did isolated compounds. Among the isolated compounds, the effectiveness in reduction of the frequency of micronuclei followed the order 4>3>2>1. The anti-lipoperoxidant activity was in the order 2>4>1, while 3 slightly increased the level of malondialdehyde. In addition, the effectiveness in induction of apoptosis followed the order, 3>2>4, while 1 had no proapoptotic effect. These results suggest that the antioxidative properties of the polyphenols tested may contribute to the radioprotective effects of G. austriaca.
本研究的目的是确定奥地利假龙胆中负责降低体外照射淋巴细胞微核发生率的活性成分。使用微核试验测定了乙醚提取物（EF）、甲醇提取物（MeF）、水溶性氧杂蒽酮去甲基雏菊叶龙胆酮（1）、去甲基雏菊叶龙胆酮8 - O - 葡萄糖苷（2）、雏菊叶龙胆酮8 - O - 葡萄糖苷（3）以及黄酮类化合物獐牙菜苷（4）对γ射线诱导的染色体损伤的辐射防护作用。EF和MeF在γ射线照射后对人淋巴细胞的处理中显示出比分离出的化合物更好的保护作用。在分离出的化合物中，降低微核频率的有效性顺序为4>3>2>1。抗脂质过氧化活性顺序为2>4>1，而3略微增加了丙二醛水平。此外，诱导凋亡的有效性顺序为3>2>4，而1没有促凋亡作用。这些结果表明，所测试多酚的抗氧化特性可能有助于奥地利假龙胆的辐射防护作用。
