Kiran M S, Viji R I, Sameer Kumar V B, Sudhakaran P R
Department of Biochemistry, University of Kerala, Thiruvananthapuram, Kerala 695 581, India.
Biochem Biophys Res Commun. 2008 Jul 4;371(3):556-60. doi: 10.1016/j.bbrc.2008.04.108. Epub 2008 Apr 28.
Investigations were carried out to understand the molecular basis of the effect of ursolic acid on angiogenesis by analysing its effects on the expression of modulators of angiogenesis by HUVECs in culture. Treatment with ursolic acid increased the expression of adhesion molecules such as E-selectin, CD-31 and I-CAM, upregulated angiogenic growth factors such as VEGF and FGF-2 and their receptors and caused increase in the ratio of PGE(2) to PGD(2). Reversal of the effect of ursolic acid by inhibition of PI3K-Akt pathway and increase in the level of phospho Akt suggest that the ursolic acid effect is mediated through PI3K-Akt pathway.
通过分析熊果酸对培养的人脐静脉内皮细胞（HUVECs）血管生成调节因子表达的影响，开展了相关研究以了解其对血管生成作用的分子基础。用熊果酸处理可增加黏附分子如E-选择素、CD-31和细胞间黏附分子（I-CAM）的表达，上调血管生成生长因子如血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（FGF-2）及其受体，并导致前列腺素E2（PGE2）与前列腺素D2（PGD2）的比值增加。抑制磷脂酰肌醇-3激酶-蛋白激酶B（PI3K-Akt）途径可逆转熊果酸的作用，且磷酸化Akt水平升高，这表明熊果酸的作用是通过PI3K-Akt途径介导的。
