Denker Elsa, Chatonnet Arnaud, Rabet Nicolas
Equipe Evolution et Développement, UMR 7138, Université Pierre et Marie Curie, Paris, France.
Chem Biol Interact. 2008 Sep 25;175(1-3):125-8. doi: 10.1016/j.cbi.2008.03.004. Epub 2008 Mar 21.
Cholinesterase activity is known in representatives of all living organisms phyla but the origin of the cholinergic system as known in bilaterian animals is still undeciphered. In particular the implication of cholinesterases in the nervous system of non-bilaterian Metazoa is not well known. We thus chose to investigate this activity in the Clytia hemisphaerica (Cnidaria) medusa. In toto histochemical staining revealed an acetylcholinesterase activity in the tentacle bulbs but not in the nervous system. Sequences homologous to acetylcholinesterase were searched within Clytia ESTs and compared to other sequences found in public databases.
所有生物门类的代表中都存在胆碱酯酶活性，但两侧对称动物中熟知的胆碱能系统的起源仍未被破解。尤其在非两侧对称后生动物的神经系统中，胆碱酯酶的作用尚不为人所知。因此，我们选择在半侧球栉水母（钵水母纲）中游水母阶段研究这种活性。整体组织化学染色显示，触手球中有乙酰胆碱酯酶活性，但神经系统中没有。我们在栉水母的ESTs中搜索了与乙酰胆碱酯酶同源的序列，并与公共数据库中找到的其他序列进行了比较。
