Jeon Won Kyung, Kim Yeong Eun, Park Sun Ok, Kwon Dae Young, Ahn Sang Woo, Lee Jeong Hwa, Ji Myoung Soon, Ko Byoung Seob
Department of Herbal Resources Research, Korea Institute of Oriental Medicine, South Korea.
Thromb Res. 2008;122(6):804-9. doi: 10.1016/j.thromres.2008.03.002. Epub 2008 Apr 29.
We investigated the in vitro effect of modified Je-Ho-Tang (MJHT) hot-water extract on platelet aggregation and activation induced by agonists in human whole blood, and on platelet adhesion to a collagen-coated surface under flow shear stress conditions. MJHT extract potently inhibited the collagen-induced platelet aggregation in human whole blood in a dose-dependent manner, with a half-maximal inhibitory dose (IC(50)) value of 526 microg/mL. We accessed several markers of platelet activation using receptor expression on platelet membranes, including GPIIb/IIIa-like expression (PAC-1) and P-selectin (CD62); we monitored the intracellular calcium mobilization response by flow cytometry in healthy subjects. A significant decrease in PAC-1 (P=0.002), CD62 (P=0.002), and intracellular calcium mobilization (P<0.001) were seen in the presence of MJHT extract. In addition, we have used image analysis to study human platelet adhesion to collagen under physiologic flow conditions in a perfusion chamber. MJHT extract markedly decreased platelet adhesion to the collagen-coated surface. These results show that MJHT has potent anti-platelet activity.
我们研究了改良柴胡汤（MJHT）热水提取物对人全血中激动剂诱导的血小板聚集和活化的体外作用，以及在流动剪切应力条件下血小板对胶原包被表面的黏附作用。MJHT提取物以剂量依赖性方式有效抑制人全血中胶原诱导的血小板聚集，半数最大抑制剂量（IC50）值为526μg/mL。我们通过血小板膜上的受体表达来检测几种血小板活化标志物，包括类GPIIb/IIIa表达（PAC-1）和P-选择素（CD62）；我们通过流式细胞术监测健康受试者的细胞内钙动员反应。在MJHT提取物存在的情况下，PAC-1（P = 0.002）、CD62（P = 0.002）和细胞内钙动员（P < 0.001）均显著降低。此外，我们利用图像分析研究了生理流动条件下灌注室中人类血小板对胶原的黏附。MJHT提取物显著降低了血小板对胶原包被表面的黏附。这些结果表明MJHT具有强大的抗血小板活性。
