Zhang Lian-Hui, Li Dong, Wang Li-Jun, Wang Ti-Peng, Zhang Lu, Chen Xiao Dong, Mao Zhi-Huai
College of Engineering, China Agricultural University, 17 Qinghua Donglu, Beijing 100083, China.
Bioresour Technol. 2008 Nov;99(17):8512-5. doi: 10.1016/j.biortech.2008.03.028. Epub 2008 Apr 29.
The effect of steam explosion pretreatment on biodegradation of lignin in wheat straw was studied in this paper. Through experiments and analysis, 0.8MPa operation pressure and 1:20 wheat straw to water ratio are optimum for destroying lignin and the maximum of lignin loss rate is 19.94%. After steam explosion pretreatment, the wheat straw was retted by Trametes versicolor for 40 days. Biodegradation rate of lignin was tested and the maximum of 55.40% lignin loss rate was found on day 30. During the whole process of both steam explosion pretreatment and biodegradation, 75.34% lignin was degraded, without steam explosion the biodegradation of raw material the degradation rate of lignin was 31.23% only. FT-IR spectroscopy, TGA and SEM were used for further validating the results of biodegradation.
本文研究了蒸汽爆破预处理对小麦秸秆中木质素生物降解的影响。通过实验与分析，0.8MPa的操作压力和1:20的小麦秸秆与水比例最有利于破坏木质素，木质素损失率最高可达19.94%。蒸汽爆破预处理后，用云芝对小麦秸秆进行40天的沤制。测试了木质素的生物降解率，在第30天发现木质素损失率最高可达55.40%。在蒸汽爆破预处理和生物降解的整个过程中，75.34%的木质素被降解，未经蒸汽爆破预处理的原料生物降解中木质素降解率仅为31.23%。利用傅里叶变换红外光谱（FT-IR）、热重分析（TGA）和扫描电子显微镜（SEM）进一步验证了生物降解结果。
