Forest and Landscape, University of Copenhagen, DK-1958, Fredriksberg C, Denmark.
Bioresour Technol. 2010 May;101(9):3175-81. doi: 10.1016/j.biortech.2009.12.008. Epub 2010 Jan 6.
The objective of the study was to characterize and map changes in lignin during hydrothermal and wet explosion pre-treatments of wheat straw and corn stover. Chemical composition, microscopic (atomic force microscopy and scanning electron microscopy) and spectroscopic (attenuated total reflectance Fourier transform infrared spectroscopy, ATR-FTIR) analyses were performed. Results showed that both pre-treatments improved the cellulose and lignin content with substantial removal of hemicellulose in the pre-treated biomasses. These values were slightly higher for hydrothermal compared to wet explosion pre-treatment. ATR-FTIR analyses also confirmed these results. Microscopic analysis showed that pre-treatments affected the biomass by partial difibration. Lignin deposition on the surface of the hydrothermally pre-treated fibre was very distinct while severe loss of fibril integrity was noticed with wet exploded fibre. The present study thus revealed that the lignin cannot be removed by the studied pre-treatments. However, both pre-treatments improved the accessibility of the biomass towards enzymatic hydrolysis.
本研究的目的是描述和绘制在小麦秸秆和玉米秸秆的水热预处理和湿式爆炸预处理过程中木质素的变化情况。进行了化学组成、微观（原子力显微镜和扫描电子显微镜）和光谱学（衰减全反射傅里叶变换红外光谱，ATR-FTIR）分析。结果表明，两种预处理方法都提高了纤维素和木质素的含量，同时大量去除了预处理生物质中的半纤维素。与湿式爆炸预处理相比，水热预处理的这些值略高。ATR-FTIR 分析也证实了这些结果。微观分析表明，预处理通过部分解纤作用影响生物质。水热预处理纤维表面的木质素沉积非常明显，而湿式爆炸纤维的原纤完整性严重损失。因此，本研究表明，研究中的预处理方法并不能去除木质素。然而，两种预处理方法都提高了生物质对酶水解的可及性。
