Talafha A Q, Lafi S Q, Ababneh M M
Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid 22110, Jordan.
Trop Anim Health Prod. 2008 Apr;40(3):185-92. doi: 10.1007/s11250-007-9079-0.
An experiment was conducted to examine the effect of estrus synchronization protocols and steroid hormones concentrations on somatic cell count (SCC) of transitional-anestrus local-Damascus cross goats' milk. Fifty-six goats (2-4-year old) were randomly assigned to three groups: fluorogestone acetate (FGA, n = 19), FGA-Prostaglandin (FGA-PGF, n = 19) and control (n = 18) groups. Intravaginal sponge containing 40 mg FGA was inserted for 13 days and an injection of 600 IU equine chorionic gonadotropin (eCG) was administered for goats of FGA and FGA-PGF groups at the time of sponge removal (day 0). In addition, goats of FGA-PGF group were injected with 10 mg dinoprost tromethamine (PGF2alpha) on day 0. Five fertile local-Damascus cross bucks were turned-in with all goats on day 0. Blood and milk samples were collected from all goats on days -13 (beginning of experiment), -6, 0, 1, 2, 7, 13 and 20 (end of the experiment). Four-year old and second-parity goats had significantly higher (p < 0.05) SCC of both udder halves than 2- and 3-year old and first-parity goats, respectively. There was a significant effect (p < 0.05) for treatment and number of kids born in the last kidding season on SCC of both udder halves. Neither estradiol nor progesterone concentrations were correlated with SCC in goats in this experiment. The SCC of both udder halves and left udder halves in goats of the control and FGA groups, respectively, increased significantly (p < 0.05) after sponge removal and buck introduction when compared with day 0, with no differences in the FGA-PGF group. This increase in SCC of the control and FGA groups coincided with peak estrus behavior. However, SCC was far below the upper limit of the current standard for normal milk. In conclusion, induction of estrus with progestagen based programs and buck introduction may cause temporary significant increase in SCC. However, the SCC values during this period of temporary increase were still in the range of acceptable values for normal milk. With the current standards for SCC of 1,000,000/ml as legal limit for abnormal milk control programs in goats, estrus synchronization programs and the estrus status should not be considered when bulk-tank milk SCC is being investigated.
进行了一项实验，以研究发情同步方案和类固醇激素浓度对处于过渡-乏情期的本地-大马士革杂交山羊奶体细胞计数（SCC）的影响。56只山羊（2至4岁）被随机分为三组：醋酸氟孕酮（FGA，n = 19）组、FGA-前列腺素（FGA-PGF，n = 19）组和对照组（n = 18）。将含有40 mg FGA的阴道海绵栓插入13天，并在取出海绵栓时（第0天）对FGA组和FGA-PGF组的山羊注射600 IU马绒毛膜促性腺激素（eCG）。此外，FGA-PGF组的山羊在第0天注射10 mg氯前列醇钠（PGF2α）。在第0天，将五只可育的本地-大马士革杂交公羊放入所有山羊群中。在实验开始的第-13天、-6天、0天、1天、2天、7天、13天和实验结束的第20天，从所有山羊采集血液和乳汁样本。4岁且第二胎的山羊两个乳房半侧的SCC分别显著高于2岁和3岁且第一胎的山羊（p < 0.05）。处理方式和上一胎产羔数对两个乳房半侧的SCC有显著影响（p < 0.05）。在本实验中，山羊体内的雌二醇和孕酮浓度与SCC均无相关性。与第0天相比，对照组和FGA组山羊两个乳房半侧及左侧乳房半侧的SCC在取出海绵栓并引入公羊后均显著增加（p < 0.05），而FGA-PGF组无差异。对照组和FGA组SCC的这种增加与发情行为高峰期一致。然而，SCC远低于当前正常牛奶标准的上限。总之，基于孕激素的发情诱导方案和引入公羊可能会导致SCC暂时显著增加。然而，在这一暂时增加期间的SCC值仍处于正常牛奶可接受值范围内。以目前山羊异常牛奶控制方案中1,000,000/ml的SCC法定限值标准来看，在调查储奶罐牛奶SCC时，不应考虑发情同步方案和发情状态。
