粪便中存在包囊和/或滋养体的肠道阿米巴病患者体内抗溶组织内阿米巴抗体。

Antibodies against Entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces.

作者信息

Carvalho M das G, Guimaräes S, Silva E F

机构信息

Department of Clinical and Toxicologycal Analyses, Pharmacy Faculty, Federal Minas Gerais University, Brazil.

出版信息

Rev Inst Med Trop Sao Paulo. 1991 Sep-Oct;33(5):379-83. doi: 10.1590/s0036-46651991000500007.

DOI:10.1590/s0036-46651991000500007

PMID:1844965

Abstract

Serum samples were obtained from 154 individuals infected with Entamoeba histolytica (78 symptomatic and 76 asymptomatic). Twelve had trophozoites in the feces whereas 142 had only cysts. The sera were used to test the existence of antibodies anti-Entamoeba histolytica employing the Indirect Hemagglutination (IHA), Indirect Immunofluorescence (IFAT), Complement Fixation Reaction (CFR) and Counterimmunoelectrophoresis (CIEP). For those individuals with trophozoites in their feces, 75.0 were positive by IHA and IFAT, 83.0 by CFR and 41.7 by CIEP. In individuals who had only cysts, positive results by the same tests were respectively, 5.6%, 12.0%, 19.0% and 5.6%. The difference in relation to the titers of antibodies detected through IHA, IFAT, CFR and CIEP and in relation to the presence of trophozoites or cysts in the feces was significative for four immunological reactions when chi 2, was employed (P < 0.05).

摘要

从154名感染溶组织内阿米巴的个体（78例有症状者和76例无症状者）采集血清样本。12人的粪便中有滋养体，而142人仅有包囊。采用间接血凝试验（IHA）、间接免疫荧光试验（IFAT）、补体结合反应（CFR）和对流免疫电泳（CIEP）检测血清中抗溶组织内阿米巴抗体的存在情况。对于粪便中有滋养体的个体，IHA和IFAT检测的阳性率为75.0%，CFR为83.0%，CIEP为41.7%。对于仅有包囊的个体，相同检测方法的阳性率分别为5.6%、12.0%、19.0%和5.6%。当采用卡方检验时，通过IHA、IFAT、CFR和CIEP检测到的抗体滴度以及粪便中滋养体或包囊的存在情况之间的差异对于四种免疫反应具有显著性（P < 0.05）。