部分纯化钩端螺旋体抗原的分离与鉴定

Isolation and characterization of partially purified leptospiral antigens.

作者信息

Koury M C, Rangel H A

机构信息

Department of Microbiology, ICB/UFMg, Belo Horizonte, Brazil.

出版信息

Rev Inst Med Trop Sao Paulo. 1991 Sep-Oct;33(5):391-6. doi: 10.1590/s0036-46651991000500009.

DOI:10.1590/s0036-46651991000500009

PMID:1844967

Abstract

The methanol extract of Leptospira interrogans serovar canicola was purified by precipitation with acetone or acetone and chloroform. The antigenicity of the antigen was not altered by heating or treatment with pepsin and pronase. However the antigenicity was lost when the antigen was treated with periodic acid. Chemical analysis revealed the presence of 40% carbohydrate (22% methylpentose, 28% hexoses), 4% protein, 20% lipid and 2.7% phosphate. The complement fixation test with sera from patients with leptospirosis agreed with the microscopic agglutination reaction.

摘要

问号钩端螺旋体犬型变种的甲醇提取物通过用丙酮或丙酮与氯仿沉淀进行纯化。该抗原的抗原性不会因加热或用胃蛋白酶和链霉蛋白酶处理而改变。然而，当抗原用高碘酸处理时，抗原性丧失。化学分析表明存在40%的碳水化合物（22%甲基戊糖、28%己糖）、4%的蛋白质、20%的脂质和2.7%的磷酸盐。用钩端螺旋体病患者血清进行的补体结合试验与显微镜凝集反应结果一致。