Fathalla N C, Coghlan J D
J Med Microbiol. 1980 Nov;13(4):513-26. doi: 10.1099/00222615-13-4-513.
Sodium dodecyl sulphate extracts of the reference strains Mus 127, Castellón 3 and Arborea of the Ballum agglutinogenic serogroup of Leptospira interrogans (the species of pathogenic leptospira), of strain Patoc 1 of the saprophytic species of L. biflexa, and of strain 3055 of illini serotype, the sole representative of L. illini, were each fractionated by ultracentrifugation in a sucrose density gradient into 10 fractions. The fractions were tested by complement fixation and immunodiffusion against the sera of animals during the process of immunisation and during the course of naturally occurring infections. The fractions could be divided into three main pools of serological activity: pool I (fractions 1, 2 and 3), pool II (fractions 4, 5 and 6), pool III (fractions 7, 8 and 9). Pool I was species/genus specific; fraction 1 tended to be species specific while fraction 2 reacted with antisera to all strains whether pathogenic or saprophytic. Pool II was serogroup specific and reacted only with antisera to members of the same serogroup. Pool III was serotype (serovar) specific and revealed the identity of the infecting strain at an early stage of infection.
问号钩端螺旋体（致病性钩端螺旋体物种）的巴伦凝集原血清群的参考菌株Mus 127、卡斯特利翁3和阿尔博雷亚，双曲钩端螺旋体腐生物种的菌株Patoc 1，以及伊利尼血清型的唯一代表菌株3055（伊利尼钩端螺旋体）的十二烷基硫酸钠提取物，分别通过在蔗糖密度梯度中进行超速离心，分成10个级分。在免疫过程中和自然感染过程中，利用补体结合试验和免疫扩散法，针对动物血清对这些级分进行检测。这些级分可分为三个主要的血清学活性池：池I（级分1、2和3），池II（级分4、5和6），池III（级分7、8和9）。池I具有种/属特异性；级分1倾向于具有种特异性，而级分2与针对所有菌株（无论是致病性还是腐生性）的抗血清发生反应。池II具有血清群特异性，仅与针对同一血清群成员的抗血清发生反应。池III具有血清型（血清变种）特异性，并在感染早期揭示感染菌株的身份。
