Yasuda P H, Sakata E E, Shikanai-Yasuda M A, Vasconcelos S de A, Romero E C, da Silva M V, Carrasco S
Instituto de Ciêncías Biomédicas da USP, Depto. de Microbiología, Brasil.
Rev Inst Med Trop Sao Paulo. 1991 Nov-Dec;33(6):497-502.
Counterimmunoelectrophoresis (CIE) was applied on paired sera from 135 patients with leptospirosis and on 69 sera from a control group. The sera from patients were subdivided in 4 groups according to the results obtained by the Microscopic Agglutination Test (MAT). The first samples sera from 58 patients were non reagent by MAT. Six monthly samples of sera were taken from 7 patients to follow-up and to determine the level of agglutinin and precipitin antibodies present using MAT and CIE. Serovars icterohaemorrhagiae and patoc were used as antigens. Three types of antigens were compared, 1) Triton-X-100 extracted; 2) heat extracted and 3) a pool of them. The CIE using icterohaemorrhagiae derived antigens types agreed with MAT in 92.64, 92.64 and 94.11% of the leptospirosis sera. The patoc antigens types reacted with the control group in 7.24, 86.95 and 84.05% of the samples, and consequently were eliminated from the present study. The icterohaemorrhagiae CIE reaction become positive earlier than MAT negative sera, and reverted to negative earlier in the follow-up samples from the patients. The CIE was sensitive and specific, gave rapid results and was easy to perform.
对流免疫电泳（CIE）应用于135例钩端螺旋体病患者的配对血清以及69例对照组血清。根据显微镜凝集试验（MAT）的结果，将患者血清分为4组。58例患者的首批样本血清MAT检测无反应。从7例患者中每月采集6份血清样本进行随访，并使用MAT和CIE检测凝集素和沉淀素抗体水平。黄疸出血型和波摩那群血清型用作抗原。比较了三种类型的抗原，1）Triton-X-100提取的；2）热提取的；3）两者的混合。使用黄疸出血型衍生抗原的CIE与钩端螺旋体病血清的MAT结果相符率分别为92.64%、92.64%和94.11%。波摩那群抗原与对照组样本的反应率分别为7.24%、86.95%和84.05%，因此从本研究中排除。黄疸出血型CIE反应比MAT阴性血清更早呈阳性，且在患者的随访样本中更早恢复为阴性。CIE灵敏、特异，结果快速且易于操作。
