Myers D M
J Clin Microbiol. 1987 May;25(5):897-9. doi: 10.1128/jcm.25.5.897-899.1987.
The counterimmunoelectrophoresis (CIE) technique employing a soluble extract antigen was found useful in screening human sera for antileptospiral antibodies to multiple leptospiral serovars. The CIE test results with sera from patients with suspected cases of leptospirosis and sera from a control group compared favorably (97.9%) with those obtained by the microscopic agglutination test using a battery of live leptospiral antigens. CIE is easy to perform, is highly sensitive, uses an antigen which is very stable at room temperature, and permits the screening of many sera in a short period of time. The CIE technique appears to be ideally suited to a small laboratory where facilities may be too limited for examining sera by the microscopic agglutination test.
采用可溶性提取物抗原的对流免疫电泳（CIE）技术，被发现可用于筛查人血清中针对多种钩端螺旋体血清型的抗钩端螺旋体抗体。对疑似钩端螺旋体病患者血清和对照组血清进行CIE检测的结果，与使用一组活钩端螺旋体抗原进行显微镜凝集试验所获得的结果相比，符合率良好（97.9%）。CIE操作简便、灵敏度高、使用的抗原在室温下非常稳定，并且能够在短时间内筛查大量血清。CIE技术似乎非常适合小型实验室，因为在这类实验室中，可能因设施有限而无法通过显微镜凝集试验检测血清。
