在钩端螺旋体病显微镜凝集试验（MAT）中使用甲醛 - PBS进行血清稀释。

Use of formaldehyde-PBS for serum dilution in the microscopic agglutination test (MAT) for leptospirosis.

作者信息

Schönberg A, Staak C, Kämpe U

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1984 Sep;257(4):493-7.

Abstract

Altogether, 431 sera (381 positive and 50 negative sera) were tested against different Leptospira serovars in the microscopic agglutination test (CMAT) using PBS with and without formaldehyde for comparative purposes. For the preparation of serum dilutions with formaldehyde-PBS, formaldehyde was added to PBS at a final concentration of 0.4%. When retested after storage, 234 from the 381 formerly positive sera gave positive, 68 doubtful and 79 negative results in the MAT. Out of the 234 sera with positive reaction in MAT, 212 (90.6%) showed positive reactions in the MAT with formaldehyde as well, 19 (8.1%) doubtful reactions and 3 (1.3%) became negative. All sera with negative reaction in the routine MAT were found to be negative in the MAT with formaldehyde as well.

摘要

总共，使用含甲醛和不含甲醛的磷酸盐缓冲盐水（PBS），通过显微镜凝集试验（CMAT）对431份血清（381份阳性血清和50份阴性血清）进行了针对不同钩端螺旋体血清型的检测，以作比较。为了用甲醛-PBS制备血清稀释液，将甲醛添加到PBS中，最终浓度为0.4%。储存后重新检测时，381份先前阳性血清中的234份在显微镜凝集试验（MAT）中呈阳性，68份结果可疑，79份呈阴性。在MAT中呈阳性反应的234份血清中，212份（90.6%）在使用甲醛的MAT中也呈阳性反应，19份（8.1%）结果可疑，3份（1.3%）变为阴性。在常规MAT中呈阴性反应的所有血清在使用甲醛的MAT中也均为阴性。