Alvarez J M, Oshima A, Mozer V, Guimarães L, Menezes H
Departamento de Imunologia, Universidade de São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 1991 Nov-Dec;33(6):509-14. doi: 10.1590/s0036-46651991000600013.
We have evaluated the sensitivity of the classical blood subinoculation method, modified through cyclophosphamide treatment of transferred mice, for the detection of occult parasitaemias in Trypanosoma cruzi chronically infected mice. Besides its simplicity, the method was shown to be highly sensitive for both the "chronic" phase parasites (99% of chronic cases were shown to harbour occult parasitaemias) and for the acute phase parasites (T. cruzi could be detected in 53.8% of animals transferred with one Y strain parasite and in 20% of animals transferred with one CL strain parasite). Using acute phase bloodforms, the assay proved to be more sensitive than conventional subinoculation when dealing with the CL, but not the Y strain of the parasite. With the help of this parasite detection tool, we have studied during a one year period, the evolution of subpatent parasitaemias in a group of mice which survived through chemotherapy from lethal acute phase of T. cruzi infection. Cyclophosphamide transfer assay revealed occult parasitaemias in 100% of the chronic animals, nevertheless, continuous and discontinuous patterns of positivity were observed.
我们评估了通过对转移小鼠进行环磷酰胺处理而改良的经典血液传代接种方法，用于检测克氏锥虫慢性感染小鼠隐匿性寄生虫血症的敏感性。除了操作简单外，该方法对“慢性”期寄生虫（99%的慢性病例显示存在隐匿性寄生虫血症）和急性期寄生虫均高度敏感（在用一株Y株寄生虫转移的动物中，53.8%可检测到克氏锥虫，在用一株CL株寄生虫转移的动物中，20%可检测到）。使用急性期血样时，该检测方法在检测CL株寄生虫时比传统传代接种更敏感，但对Y株寄生虫则不然。借助这种寄生虫检测工具，我们在一年时间里研究了一组从克氏锥虫感染致死急性期经化疗存活下来的小鼠亚临床寄生虫血症的演变情况。环磷酰胺转移试验显示100%的慢性动物存在隐匿性寄生虫血症，不过，观察到了持续和间断的阳性模式。
