Dimock K A, Davis C D, Kuhn R E
Department of Biology, Wake Forest University, Winston-Salem, North Carolina 27109.
Infect Immun. 1991 Dec;59(12):4377-82. doi: 10.1128/iai.59.12.4377-4382.1991.
When held at 36 degrees C, Trypanosoma cruzi-infected C3H mice survive an otherwise lethal infection with significantly decreased parasitemia levels and enhanced immune responsiveness. Treatment of T. cruzi-infected mice with the immunosuppressive agent cyclophosphamide indicated that the positive effects of increased environmental temperature were primarily due to enhancement of immunity. A parasite-specific, enzyme-linked immunosorbent assay and immunoblot analysis were used to examine the effect of elevated environmental temperature on the production of anti-T. cruzi antibodies. Both the reactivity and diversity of anti-T. cruzi antibodies were found to be lower in infected mice held at 36 degrees C than in infected mice held at room temperature. However, reactivity and diversity could be enhanced by vaccination with culture forms of the parasite.
当饲养在36摄氏度时，感染克氏锥虫的C3H小鼠能在原本致命的感染中存活下来，寄生虫血症水平显著降低，免疫反应性增强。用免疫抑制剂环磷酰胺治疗感染克氏锥虫的小鼠表明，环境温度升高的积极作用主要是由于免疫力增强。采用寄生虫特异性酶联免疫吸附测定和免疫印迹分析来检测环境温度升高对抗克氏锥虫抗体产生的影响。结果发现，饲养在36摄氏度的感染小鼠体内，抗克氏锥虫抗体的反应性和多样性均低于饲养在室温下的感染小鼠。然而，通过接种寄生虫培养形式的疫苗可增强反应性和多样性。
