小鼠中枢神经系统发育过程中的微小RNA表达谱
MicroRNA expression profile in murine central nervous system development.
作者信息
Dogini Danyella B, Ribeiro Patrícia A O, Rocha Cristiane, Pereira Tiago C, Lopes-Cendes Iscia
机构信息
Department of Medical Genetics, Faculty of Medical Sciences, University of Campinas-UNICAMP, Tessália Vieira de Camargo 126, Campinas, Sao Paulo, Brazil.
出版信息
J Mol Neurosci. 2008 Jul;35(3):331-7. doi: 10.1007/s12031-008-9068-4. Epub 2008 May 2.
MicroRNAs (miRNAs) regulate gene expression in a post-transcriptional sequence-specific manner. In order to better understand the possible roles of miRNAs in central nervous system (CNS) development, we examined the expression profile of 104 miRNAs during murine brain development. We obtained brain samples from animals at embryonic days (E) E15, E17, and postnatal days (P) P1 and P7. Total RNA was isolated from tissue and used to obtain mature miRNAs by reverse transcription. Our results indicate that there is a group of 12 miRNAs that show a distinct expression profile, with the highest expression during embryonic stages and decreasing significantly during development. This profile suggests key roles in processes occurring during early CNS development.
微小RNA（miRNA）以转录后序列特异性方式调节基因表达。为了更好地理解miRNA在中枢神经系统（CNS）发育中的可能作用，我们检测了104种miRNA在小鼠脑发育过程中的表达谱。我们从胚胎期（E）E15、E17以及出生后（P）P1和P7的动物获取脑样本。从组织中分离总RNA，并通过逆转录获得成熟的miRNA。我们的结果表明，有一组12种miRNA呈现出独特的表达谱，在胚胎阶段表达最高，在发育过程中显著下降。这种表达谱表明它们在中枢神经系统早期发育过程中发挥关键作用。
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