Joshua Anthony M, Boutros Paul C
Department of Medical Oncology, Princess Margaret Hospital, Toronto, Canada.
Methods Mol Med. 2008;141:309-29. doi: 10.1007/978-1-60327-148-6_17.
In the post-Human Genome Project era, awareness of the resources available through the internet is essential to both molecular biologists and clinicians. An overview of the main databases and analytical tools described in this chapter is important to understand the principles upon which hypotheses are generated, experiments are based and conclusions reached. Similarly, an introduction to the terminology of these resources often facilitates their use and adoption into practice. This chapter covers database resources such as NCBI/ Entrez, Ensembl and UCSC as well as analytical tools for sequence alignment, promoter analysis and molecular interactions.
在后人类基因组计划时代,了解通过互联网可获取的资源对于分子生物学家和临床医生而言都至关重要。本章中所描述的主要数据库和分析工具的概述,对于理解生成假设、开展实验以及得出结论所依据的原理很重要。同样,介绍这些资源的术语通常有助于其在实际中的使用和应用。本章涵盖了诸如NCBI/Entrez、Ensembl和UCSC等数据库资源,以及用于序列比对、启动子分析和分子相互作用的分析工具。