Kin Nicholas W, Crawford Dianna M, Liu Jiabin, Behrens Timothy W, Kearney John F
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294.
Center for Immunology, Department of Medicine, University of Minnesota, Minneapolis, MN 55455.
J Immunol. 2008 May 15;180(10):6663-6674. doi: 10.4049/jimmunol.180.10.6663.
Marginal zone (MZ) B cells play an important role in the clearance of blood-borne bacterial infections via rapid T-independent IgM responses. We have previously demonstrated that MZ B cells respond rapidly and robustly to bacterial particulates. To determine the MZ-specific genes that are expressed to allow for this response, MZ and follicular (FO) B cells were sort purified and analyzed via DNA microarray analysis. We identified 181 genes that were significantly different between the two B cell populations. Ninety-nine genes were more highly expressed in MZ B cells while 82 genes were more highly expressed in FO B cells. To further understand the molecular mechanisms by which MZ B cells respond so rapidly to bacterial challenge, Id-positive and -negative MZ B cells were sort purified before (0 h) or after (1 h) i.v. immunization with heat-killed Streptococcus pneumoniae, R36A, and analyzed via DNA microarray analysis. We identified genes specifically up-regulated or down-regulated at 1 h following immunization in the Id-positive MZ B cells. These results give insight into the gene expression pattern in resting MZ vs FO B cells and the specific regulation of gene expression in Ag-specific MZ B cells following interaction with Ag.
边缘区（MZ）B细胞通过快速的非依赖T细胞的IgM反应在清除血源细菌感染中发挥重要作用。我们之前已经证明MZ B细胞对细菌颗粒有快速且强烈的反应。为了确定为实现这种反应而表达的MZ特异性基因，对MZ和滤泡（FO）B细胞进行了分选纯化，并通过DNA微阵列分析进行了检测。我们鉴定出在这两种B细胞群体之间存在显著差异的181个基因。99个基因在MZ B细胞中表达更高，而82个基因在FO B细胞中表达更高。为了进一步了解MZ B细胞对细菌攻击反应如此迅速的分子机制，在用热灭活的肺炎链球菌R36A进行静脉内免疫之前（0小时）或之后（1小时），对Id阳性和阴性的MZ B细胞进行了分选纯化，并通过DNA微阵列分析进行了检测。我们鉴定出在Id阳性MZ B细胞免疫后1小时特异性上调或下调的基因。这些结果深入了解了静息MZ与FO B细胞中的基因表达模式，以及与抗原相互作用后抗原特异性MZ B细胞中基因表达的特定调控。
