González-Agüero Mauricio, Pavez Leonardo, Ibáñez Freddy, Pacheco Igor, Campos-Vargas Reinaldo, Meisel Lee A, Orellana Ariel, Retamales Julio, Silva Herman, González Mauricio, Cambiazo Verónica
Laboratorio de Bioinformática y Expresión Génica, INTA-Universidad de Chile, Millennium Nucleus Center for Genomics of the Cell (CGC), Santiago, Chile.
J Exp Bot. 2008;59(8):1973-86. doi: 10.1093/jxb/ern069. Epub 2008 May 3.
Woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods of cold storage. This disorder is of commercial importance since shipping of peaches to distant markets and storage before selling require low temperature. However, knowledge about the molecular basis of peach woolliness is still incomplete. To address this issue, a nylon macroarray containing 847 non-redundant expressed sequence tags (ESTs) from a ripe peach fruit cDNA library was developed and used. Gene expression changes of peach fruit (Prunus persica cv. O'Henry) ripened for 7 d at 21 degrees C (juicy fruit) were compared with those of fruit stored for 15 d at 4 degrees C and then ripened for 7 d at 21 degrees C (woolly fruit). A total of 106 genes were found to be differentially expressed between juicy and woolly fruit. Data analysis indicated that the activity of most of these genes (>90%) was repressed in the woolly fruit. In cold-stored peaches (cv. O'Henry), the expression level of selected genes (cobra, endopolygalacturonase, cinnamoyl-CoA-reductase, and rab11) was lower than in the juicy fruit, and it remained low in woolly peaches after ripening, a pattern that was conserved in woolly fruit from two other commercial cultivars (cv. Flamekist and cv. Elegant Lady). In addition, the results of this study indicate that molecular changes during fruit woolliness involve changes in the expression of genes associated with cell wall metabolism and endomembrane trafficking. Overall, the results reported here provide an initial characterization of the transcriptome activity of peach fruit under different post-harvest treatments.
发绵是桃和油桃的一种生理病害，当果实经过长时间冷藏后再成熟时就会显现出来。这种病害具有商业重要性，因为将桃子运往遥远的市场以及销售前的储存都需要低温。然而，关于桃发绵的分子基础的知识仍然不完整。为了解决这个问题，开发并使用了一种尼龙宏阵列，它包含来自成熟桃果实cDNA文库的847个非冗余表达序列标签（EST）。将在21摄氏度下成熟7天的桃果实（Prunus persica cv. O'Henry，多汁果实）与在4摄氏度下储存15天然后在21摄氏度下成熟7天的果实（发绵果实）的基因表达变化进行了比较。总共发现有106个基因在多汁果实和发绵果实之间差异表达。数据分析表明，这些基因中的大多数（>90%）在发绵果实中的活性受到抑制。在冷藏的桃子（cv. O'Henry）中，所选基因（眼镜蛇蛋白、内切多聚半乳糖醛酸酶、肉桂酰辅酶A还原酶和rab11）的表达水平低于多汁果实，并且在成熟后的发绵桃子中仍然较低，这种模式在另外两个商业品种（cv. Flamekist和cv. Elegant Lady）的发绵果实中也存在。此外，本研究结果表明，果实发绵过程中的分子变化涉及与细胞壁代谢和内膜运输相关的基因表达变化。总体而言，这里报道的结果提供了不同采后处理条件下桃果实转录组活性的初步特征。
