Li Grace N, Hoffman-Kim Diane
Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI 02912, USA.
Tissue Eng Part B Rev. 2008 Mar;14(1):33-51. doi: 10.1089/teb.2007.0181.
Tissue engineering provides a valuable tool for in vitro investigation of complex in vivo environments. A particular application of tissue-engineered in vitro platforms in neuroscience and regenerative medicine is the fabrication of controlled microenvironments for the study of axon guidance, with the goal of informing strategies to overcome nerve injury. The innovative design of tissue-engineered scaffolds that incorporate multiple guidance cues and cell types into various environments is advancing the understanding of how neurons integrate guidance information to make growth decisions. This review focuses on recent strategies that present neurons with multiple cues with micro- and nanoscale resolution in order to study the interactions between neurons and their local environment during axon guidance.
组织工程为体外研究复杂的体内环境提供了一个有价值的工具。组织工程体外平台在神经科学和再生医学中的一个特殊应用是制造可控的微环境以研究轴突导向，目的是为克服神经损伤的策略提供信息。将多种导向线索和细胞类型整合到各种环境中的组织工程支架的创新设计，正在推动人们对神经元如何整合导向信息以做出生长决策的理解。本综述重点关注最近的策略，这些策略以微米和纳米级分辨率为神经元提供多种线索，以便研究轴突导向过程中神经元与其局部环境之间的相互作用。
