NUS Graduate School for Integrative Sciences and Engineering, Department of Biomolecular and Chemical Engineering, National University of Singapore, Singapore.
Acta Biomater. 2012 Feb;8(2):540-8. doi: 10.1016/j.actbio.2011.09.026. Epub 2011 Sep 28.
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) microspheres, with properties such as slower degradation and more efficient drug delivery properties, have important benefits for neural tissue engineering. Our previous studies have shown PHBV microspheres to improve cell growth and differentiation. This study aimed to investigate if PHBV microspheres would support neurons to extend these benefits to neural tissue engineering. PHBV microspheres' suitability as neural tissue engineering scaffolds was investigated using PC12 cells, cortical neurons (CNs), and neural progenitor cells (NPCs) to cover a variety of neuronal types for different applications. Microspheres were fabricated using an emulsion-solvent-evaporation technique. DNA quantification, cell viability assays, and immunofluorescent staining were carried out. PC12 cultures on PHBV microspheres showed growth trends comparable to two-dimensional controls. This was further verified by staining for cell spreading. Also, CNs expressed components of the signaling pathway on PHBV microspheres, and had greater axon-dendrite segregation (4.1 times for axon stains and 2.3 times for dendrite stains) than on coverslips. NPCs were also found to differentiate into neurons on the microspheres. Overall, the results indicate that PHBV microspheres, as scaffolds for neural tissue engineering, supported a variety of neuronal cell types and promoted greater axon-dendrite segregation.
聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）微球具有降解缓慢和更有效的药物传递性能等特性，对神经组织工程具有重要意义。我们之前的研究表明 PHBV 微球能够促进细胞生长和分化。本研究旨在探讨 PHBV 微球是否能够将这些益处扩展到神经组织工程中，以支持神经元。使用 PC12 细胞、皮质神经元 (CNs) 和神经前体细胞 (NPCs) 来覆盖各种不同应用的神经元类型，研究了 PHBV 微球作为神经组织工程支架的适用性。使用乳液-溶剂蒸发技术制备微球。进行了 DNA 定量、细胞活力测定和免疫荧光染色。在 PHBV 微球上培养的 PC12 细胞表现出与二维对照相似的生长趋势。这通过细胞铺展染色进一步得到证实。此外，CNs 在 PHBV 微球上表达了信号通路的组成部分，并且与在盖玻片上相比，具有更大的轴突-树突分离（轴突染色增加 4.1 倍，树突染色增加 2.3 倍）。还发现 NPC 可以在微球上分化为神经元。总体而言，这些结果表明，作为神经组织工程支架的 PHBV 微球支持多种神经元细胞类型，并促进更大的轴突-树突分离。
