实验大鼠和技术人员中肺支原体的检测。

Detection of Mycoplasma pulmonis in laboratory rats and technicians.

作者信息

Ferreira J B, Yamaguti M, Marques L M, Oliveira R C, Neto R L, Buzinhani M, Timenetsky J

机构信息

Departamento de Microbiologia, Laboratório de Micoplasmas, Instituto de Ciências Biomédicas-USP, Av. Prof. Lineu Prestes, São Paulo, Brasil.

出版信息

Zoonoses Public Health. 2008 Jun;55(5):229-34. doi: 10.1111/j.1863-2378.2008.01122.x.

DOI:10.1111/j.1863-2378.2008.01122.x

PMID:18454744

Abstract

Five species of mycoplasma are associated with several rat diseases. Mycoplasma pulmonis is the most important and most studied, possibly causing disease in rats and undermining the validity of laboratory experiments. M. pulmonis was isolated in 144/240 laboratory rats and identified by PCR in 155/240. This species was also detected in 12 human individuals (technicians of a laboratory animal house hold) in contact with these rats. The results were confirmed by sequencing of DNA products. Mycoplasma species are host specific; however, M. pulmonis was identified in humans, suggesting a case of unspecific colonization. Statistical analysis shows a greater risk for M. pulmonis colonizing individuals who are exposed to infected rats in animal facilities than individuals who do not. The detection of M. pulmonis in humans indicates a new status for this mollicute mycoplasmas in animal-holding facilities.

摘要

五种支原体与几种大鼠疾病有关。肺支原体是最重要且研究最多的，可能导致大鼠患病并影响实验室实验的有效性。在240只实验大鼠中，144只分离出了肺支原体，155只通过聚合酶链反应（PCR）鉴定出来。在与这些大鼠接触的12名人类个体（一家实验动物房的技术人员）中也检测到了该物种。DNA产物测序证实了结果。支原体具有宿主特异性；然而，在人类中发现了肺支原体，提示存在非特异性定植的情况。统计分析表明，与未接触感染大鼠的个体相比，在动物设施中接触感染大鼠的个体感染肺支原体的风险更高。在人类中检测到肺支原体表明这种柔膜体支原体在动物饲养设施中有了新情况。