Pirev Elvis, Calles Christian, Schroeder Peter, Sies Helmut, Kröncke Klaus-D
Institute of Biochemistry and Molecular Biology I, Heinrich-Heine-University of Düsseldorf, D-40225 Düsseldorf, Germany.
Free Radic Biol Med. 2008 Jul 1;45(1):86-91. doi: 10.1016/j.freeradbiomed.2008.04.011. Epub 2008 Apr 30.
Changes of the redox balance in cells alter the availability of intracellular free Zn(2+). Here, cells were exposed to ultraviolet (UV)-A, UV-B, or infrared (IR)-A light irradiation, and the intracellular free zinc pool was monitored. Under sublethal conditions only UV-A irradiation resulted in a transient cytoplasmic and nuclear increase of intracellular free Zn(2+). Likewise, tert-butyl hydroperoxide and singlet oxygen, but not H(2)O(2) or intracellular generation of O(2)(*-) by redox cyclers, mimicked the effects of UV-A irradiation, while disulfide stress by diamide only led to a transient cytoplasmic zinc release. These results show that only certain types of subtoxic cellular stress massively disturb the zinc homeostasis in cells.
细胞中氧化还原平衡的变化会改变细胞内游离锌离子（Zn(2+)）的可利用性。在此，将细胞暴露于紫外线A（UV-A）、紫外线B（UV-B）或红外线A（IR-A）照射下，并监测细胞内游离锌池。在亚致死条件下，仅UV-A照射导致细胞内游离Zn(2+)在细胞质和细胞核中短暂增加。同样，叔丁基过氧化氢和单线态氧，但不是过氧化氢或氧化还原循环剂在细胞内产生的超氧阴离子（O(2)(*-)），模拟了UV-A照射的效果，而二酰胺引起的二硫键应激仅导致细胞质锌的短暂释放。这些结果表明，只有某些类型的亚毒性细胞应激会严重扰乱细胞内的锌稳态。
