Damous L L, Silva S M, Simões R S, Morello R J, Carbonel A P F, Simões M J, Montero E F S
Department of Surgery, Federal University of São Paulo, São Paulo, Brazil.
Transplant Proc. 2008 Apr;40(3):861-4. doi: 10.1016/j.transproceed.2008.02.065.
Verify the optimum remote vascular occlusion time to reduce ovarian injury in autologous transplants in rats.
Twenty-four adult female rats were assigned to four groups: GC (control group): bilateral oophorectomy followed by ovary transplant; GIPC (ischemic preconditioning group): remote ischemic preconditioning at the iliac artery for 5, 10, and 15 minutes (GIPC-5, GIPC-20, and GIPC-15) previous to bilateral oophorectomy and ovarian transplantation. The right ovary was fixed in the retroperitoneum. Euthanasia was performed 4 days after the surgical procedure. The follicles were counted and classified as developing versus atretic. The immunohistochemical assay identified vascular factor of endothelial growth (VEGF) in the ovarian stroma and assessed the proliferation capacity by means of the Ki-67 in the ovarian follicles.
Every group showed an inflammatory infiltrate, luteous body, and ovarian follicles in several phases of development. The ischemic preconditioning groups displayed greater amounts of viable ovarian follicles and increased vascularization and vasodilatation than the control group. GIPC-15 showed the highest amount of viable follicles compared to the others (P < .05 GIPC-15 vs GC; GIPC-15 vs GIPC-5). More VEGF-labeled cells were observed in GIPC-10 than the control group (P < .05, GIPC-10 vs GC). The proliferation index assessed by Ki-67 marking showed GC: 80%; GIPC-5: 76%; GIPC-10: 67%; and GIPC-15: 64% (P > .05).
The PCI-15 cohort seem to be the most adequate timing to achieve functional support and preservation of a greater number of viable ovarian follicles.
验证在大鼠自体移植中减少卵巢损伤的最佳远程血管闭塞时间。
将24只成年雌性大鼠分为四组：GC（对照组）：双侧卵巢切除术后进行卵巢移植；GIPC（缺血预处理组）：在双侧卵巢切除和卵巢移植前，对髂动脉进行5、10和15分钟的远程缺血预处理（GIPC - 5、GIPC - 10和GIPC - 15）。右侧卵巢固定于腹膜后。手术后4天实施安乐死。对卵泡进行计数并分为发育卵泡和闭锁卵泡。免疫组织化学分析确定卵巢基质中内皮生长血管因子（VEGF），并通过Ki - 67评估卵巢卵泡的增殖能力。
每组均显示有炎症浸润、黄体和处于几个发育阶段的卵巢卵泡。与对照组相比，缺血预处理组显示出更多的存活卵巢卵泡，血管生成和血管扩张增加。与其他组相比，GIPC - 15显示出最高数量的存活卵泡（GIPC - 15与GC相比，P <.05；GIPC - 15与GIPC - 5相比）。在GIPC - 10中观察到的VEGF标记细胞比对照组更多（GIPC - 10与GC相比，P <.05）。通过Ki - 67标记评估的增殖指数显示：GC组为80%；GIPC - 5组为76%；GIPC - 10组为67%；GIPC - 15组为64%（P >.05）。
PCI - 15组似乎是实现功能支持和保留更多存活卵巢卵泡的最合适时机。
