Damous Luciana Lamarão, Silva Sônia Maria da, Lopes Renato Antônio Migliano, Sakano Célia Regina de Souza Bezerra, Simões Manuel de Jesus, Montero Edna Frasson de Souza
Surgery and Research Postgraduate Program, UNIFESP, Sao Paulo, Brazil.
Acta Cir Bras. 2009 Sep-Oct;24(5):387-92. doi: 10.1590/s0102-86502009000500009.
To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats.
Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles.
R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05).
R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones.
通过对移植大鼠进行阴道涂片，评估远程缺血预处理（R-IPC）对卵巢移植质量的影响。
使用60只大鼠，分为六组：对照组；新鲜移植组（TxF）；冷冻保存移植组（TxC）；R-IPC组；R-IPC + 新鲜移植组（TxF+R-IPC）；R-IPC + 冷冻保存移植组（TxC+R-IPC）。在髂总动脉进行R-IPC。将自体卵巢组织完整植入腹膜后。在术后第一天开始每日收集阴道涂片。30天后，将重新开始发情周期的术后天数以及发情天数和发情周期数纳入分析。
R-IPC显示出早期发情重新开始的趋势（p>0.05），并且增加了新鲜移植组的周期数，而在冷冻保存移植组中，无论是否有R-IPC刺激，周期数相似（p>0.05）。接受新鲜移植的动物的发情期比接受冷冻保存移植的动物长，无论有无R-IPC（p<0.05）。
R-IPC促进了术后卵巢活动的更早重新开始和更高的发情频率，在新鲜移植组中的结果比冷冻保存移植组更一致。
