Sugimoto M, Maeda S, Manabe N, Miyamoto H
Laboratory of Anatomy and Cell Biology, Graduate School of Agriculture Kyoto University, Japan.
Theriogenology. 2000 Mar 15;53(5):1093-103. doi: 10.1016/S0093-691X(00)00255-7.
We cryopreserved infantile rat ovaries by vitrification and assessed their viability by autotransplantation. Hemilateral ovarian transplantation was performed on rats on postnatal Days 10 to 12. The left ovary of each rat was dissected out, cryopreserved by vitrification using a modified vitrification solution (VS1), and then autotransplanted under the capsule of the right kidney. The right ovary of each rat was removed. For the control, the left ovary was dissected out from each rat and was immediately transplanted by the same procedure, without cryopreservation. Rats were nursed until weaning, and then the day of vaginal opening, estrous cyclicity from the day of vaginal opening until postnatal Day 84, and histology of ovarian grafts at postnatal Day 84 were examined. The time course of development of endocrine function of cryopreserved grafts was similar to that of fresh grafts. In ovarian transplants recovered on postnatal Day 84, antral follicles and corpora lutea (CL) were observed in addition to small follicles, although the number of antral follicles in cryopreserved grafts was smaller than in the fresh grafts. These results indicate that cryopreservation of ovarian tissue by vitrification can be used for the preservation of fertility and endocrine function of ovaries.
我们通过玻璃化冷冻保存幼鼠卵巢，并通过自体移植评估其活力。在出生后第10至12天对大鼠进行单侧卵巢移植。解剖出每只大鼠的左卵巢，使用改良玻璃化溶液（VS1）通过玻璃化进行冷冻保存，然后自体移植到右肾包膜下。切除每只大鼠的右卵巢。作为对照，从每只大鼠中解剖出左卵巢，不经冷冻保存，立即通过相同程序进行移植。大鼠饲养至断奶，然后检查阴道开口日期、从阴道开口日至出生后第84天的发情周期以及出生后第84天卵巢移植物的组织学。冷冻保存移植物内分泌功能的发育时间进程与新鲜移植物相似。在出生后第84天恢复的卵巢移植中，除了小卵泡外，还观察到有腔卵泡和黄体（CL），尽管冷冻保存移植物中有腔卵泡的数量比新鲜移植物中的少。这些结果表明，通过玻璃化冷冻保存卵巢组织可用于保存卵巢的生育能力和内分泌功能。
