Davydova V N, Bratskaya S Yu, Gorbach V I, Solov'eva T F, Kaca W, Yermak I M
Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia.
Biophys Chem. 2008 Jul;136(1):1-6. doi: 10.1016/j.bpc.2008.03.006. Epub 2008 Apr 8.
Electrokinetic properties of complexes of chitosan (Ch) with lipopolysaccharides (LPSs) from Escherichia coli O55:B5, Yersinia pseudotuberculosis 1B 598, and Proteus vulgaris O25 (48/57) and their size distribution were investigated using zeta-potential distribution assay and quasi-elastic light scattering. The interaction of LPS from different microorganisms with chitosan at the same w/w ratio of components (1:1) resulted in the formation of complexes in which the negative charge of LPS was neutralized (LPS from E. coli) or overcompensated (Y. pseudotuberculosis and P. vulgaris). The changing in size of the endotoxin aggregates during binding with chitosan was observed. The binding constants of chitosan with LPSs were determined by a method with using the anionic dye Orange II. The LPS from E. coli possess higher affinity to chitosan in comparison with the two others samples of endotoxin.
利用ζ-电位分布测定法和准弹性光散射研究了壳聚糖（Ch）与大肠杆菌O55:B5、假结核耶尔森菌1B 598和普通变形杆菌O25（48/57）的脂多糖（LPS）形成的复合物的电动性质及其尺寸分布。不同微生物来源的LPS与壳聚糖在相同的组分质量比（1:1）下相互作用，导致形成复合物，其中大肠杆菌的LPS负电荷被中和，而假结核耶尔森菌和普通变形杆菌的LPS负电荷被过度补偿。观察到内毒素聚集体与壳聚糖结合过程中尺寸的变化。采用阴离子染料橙黄II法测定壳聚糖与LPS的结合常数。与另外两种内毒素样品相比，大肠杆菌的LPS对壳聚糖具有更高的亲和力。
