Khorrami Afshin Rajabi, Mehrseresht Shima
Department of Chemistry, Faculty of Science, Islamic Azad University, Karaj Branch, Karaj, Iran.
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 May 15;867(2):264-9. doi: 10.1016/j.jchromb.2008.04.017. Epub 2008 May 2.
A molecularly imprinted polymer (MIP) has been prepared using levonorgestrel (LEV) as template. The polymer was synthesised in a non-covalent approach using methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linking monomer via a free radical polymerization. An equivalent blank polymer was also synthesised in the absence of the template compound. Batch adsorption experiments were used to evaluate the binding affinity of the imprinted polymer. After packing MIP into a stainless steel column (150 mm x 4.6 mm i.d.), retention and elution of the template and related compounds were evaluated by high-performance liquid chromatography (HPLC). This LEV imprinted polymer was further applied for selective solid phase extraction (SPE) of LEV from human serum. It was confirmed that the binding ability of the prepared MIP for LEV was essentially sufficient in the presence of other compounds coexisting in serum sample. Therefore, as a selective and efficient solid phase material, LEV imprinted polymer has a high potential application in analysis of this steroidal hormone in clinical purposes.
以左炔诺孕酮（LEV）为模板制备了一种分子印迹聚合物（MIP）。该聚合物采用非共价法合成，以甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）为交联单体，通过自由基聚合反应制得。在不存在模板化合物的情况下，还合成了一种等效的空白聚合物。采用批量吸附实验来评估印迹聚合物的结合亲和力。将MIP填充到不锈钢柱（内径150 mm×4.6 mm）中后，通过高效液相色谱（HPLC）评估模板及相关化合物的保留和洗脱情况。这种LEV印迹聚合物进一步应用于从人血清中选择性固相萃取（SPE）LEV。结果证实，在血清样品中存在其他共存化合物的情况下，所制备的MIP对LEV的结合能力基本足够。因此，作为一种选择性高效的固相材料，LEV印迹聚合物在临床目的甾体激素分析中具有很高的潜在应用价值。
