Ma Xingbin, Lin Hongling, Yong Yanhong, Ju Xianghong, Li Youquan, Liu Xiaoxi, Yu Zhichao, Wujin Cuomu, She Yongxin, Zhang Jiyu, Abd El-Aty A M
Department of Veterinary Medicine, Guangdong Ocean University, Zhanjiang, China.
Zhanjiang Experimental Station, Southern-Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, China.
Front Nutr. 2022 Oct 21;9:950044. doi: 10.3389/fnut.2022.950044. eCollection 2022.
The minor constituent found in Linnaeus, 4-hydroxy-2 (3H) benzoxazolone alkaloid (HBOA), has a range of versatile applications. Herein, a quick and straightforward method for extracting HBOA from Linnaeus was proposed. HBOA was used as a template, whereas methacrylic acid, ethylene glycol dimethacrylate, and acetonitrile were used as functional monomers, cross-linkers, and porogens, respectively. Molecularly imprinted polymers (MIPs) were synthesized by precipitation polymerization, and their adsorption isotherms, dynamics, and selective binding ability were characterized and analyzed. The results showed that the adsorption amount of the template was 90.18 mg/g. The MIPs were used as solid-phase extraction fillers and actual sample extraction columns, with a linear range of 0-100 μg/L, average recovery of 78.50-101.12%, and a relative standard deviation of 1.20-3.26%. The HBOA concentrations in the roots, stems, and leaves were 1,226, 557, and 205 μg/g, respectively. In addition, MIP-SPE was successfully used in isolating and purifying HBOA from different parts of Linnaeus, indicating its effectiveness in extracting and determining HBOA in other herbs.
在Linnaeus中发现的次要成分4-羟基-2（3H）苯并恶唑酮生物碱（HBOA）具有一系列广泛的应用。本文提出了一种从Linnaeus中快速直接提取HBOA的方法。以HBOA为模板，分别以甲基丙烯酸、乙二醇二甲基丙烯酸酯和乙腈作为功能单体、交联剂和致孔剂。通过沉淀聚合法合成了分子印迹聚合物（MIPs），并对其吸附等温线、动力学和选择性结合能力进行了表征和分析。结果表明，模板的吸附量为90.18 mg/g。将MIPs用作固相萃取填料和实际样品萃取柱，线性范围为0-100 μg/L，平均回收率为78.50-101.12%，相对标准偏差为1.20-3.26%。根、茎和叶中HBOA的浓度分别为1226、557和205 μg/g。此外，MIP-SPE成功用于从Linnaeus的不同部位分离和纯化HBOA，表明其在提取和测定其他草药中HBOA方面的有效性。
