Rubio-Ruiz Maria Esther, Díaz-Díaz Eulises, Cárdenas-León Mario, Argüelles-Medina Rabindranath, Sánchez-Canales Patricia, Larrea-Gallo Fernando, Soria-Castro Elizabeth, Guarner-Lans Verónica
Department of Physiology, Instituto Nacional de Cardiología Ignacio Chávez, Mexico City, Mexico.
Glycobiology. 2008 Jul;18(7):517-25. doi: 10.1093/glycob/cwn034. Epub 2008 May 5.
The effects of nonglycated bovine serum albumin (BSA) and advanced glycosylation end products of BSA (AGE-BSA) on vascular responses of control and metabolic syndrome (MS) rats characterized by hypertriglyceridemia, hypertension, hyperinsulinemia, and insulin resistance were studied. Albumin and in vitro prepared AGE-BSA have vascular effects; however, recent studies indicate that some effects of in vitro prepared AGEs are due to the conditions in which they were generated. We produced AGEs by incubating glucose with BSA for 60 days under sterile conditions in darkness and at 37 degrees C. To develop MS rats, male Wistar animals were given 30% sucrose in drinking water since weanling. Six month old animals were used. Blood pressure, insulin, triglycerides, and serum albumin were increased in MS rats. Contraction of aortic rings elicited with norepinephrine was stronger. There were no effects of nonglycated BSA or AGE-BSA on contractions in control or MS rats; however, both groups responded to L-NAME, an inhibitor of nitric oxide synthesis. Arterial relaxation induced using acetylcholine was smaller in MS rats. Nonglycated BSA and AGE-BSA significantly diminished relaxation in a 35% in the control group but the decrease was similar when using nonglycated BSA and AGE-BSA. This decrease was not present in the MS rats and was not due to increased RAGEs or altered biochemical characteristics of BSA. In conclusion, both BSA and AGE-BSA inhibit vascular relaxation in control artic rings. In MS rats the effect is lost possibly due to alterations in endothelial cells that are a consequence of the illness.
研究了非糖化牛血清白蛋白（BSA）和牛血清白蛋白的晚期糖基化终产物（AGE-BSA）对以高甘油三酯血症、高血压、高胰岛素血症和胰岛素抵抗为特征的对照大鼠和代谢综合征（MS）大鼠血管反应的影响。白蛋白和体外制备的AGE-BSA具有血管效应；然而，最近的研究表明，体外制备的一些AGEs的效应是由于其产生的条件所致。我们通过在无菌条件下、黑暗中37℃将葡萄糖与BSA孵育60天来生成AGEs。为了培育MS大鼠，雄性Wistar动物自断奶起饮用含30%蔗糖的水。使用6月龄的动物。MS大鼠的血压、胰岛素、甘油三酯和血清白蛋白升高。去甲肾上腺素引起的主动脉环收缩更强。非糖化BSA或AGE-BSA对对照大鼠或MS大鼠的收缩均无影响；然而，两组均对一氧化氮合成抑制剂L-NAME有反应。MS大鼠中乙酰胆碱诱导的动脉舒张较小。非糖化BSA和AGE-BSA使对照组的舒张显著降低35%，但使用非糖化BSA和AGE-BSA时降低程度相似。这种降低在MS大鼠中不存在，且不是由于RAGEs增加或BSA的生化特性改变所致。总之，BSA和AGE-BSA均抑制对照动脉环中的血管舒张。在MS大鼠中，这种效应可能由于疾病导致的内皮细胞改变而丧失。
